Characterization of HIV-1 drug resistance among patients with failure of second-line combined antiretroviral therapy in central Ethiopia
© 2021 The Authors. HIV Medicine published by John Wiley & Sons Ltd on behalf of British HIV Association..
BACKGROUND: As a consequence of the improved availability of combined antiretroviral therapy (cART) in resource-limited countries, an emergence of HIV drug resistance (HIVDR) has been observed. We assessed the prevalence and spectrum of HIVDR in patients with failure of second-line cART at two HIV clinics in central Ethiopia.
METHODS: HIV drug resistance was analysed in HIV-1-infected patients with virological failure of second-line cART using the geno2pheno application.
RESULTS: Among 714 patients receiving second-line cART, 44 (6.2%) fulfilled the criteria for treatment failure and 37 were eligible for study inclusion. Median age was 42 years [interquartile range (IQR): 20-45] and 62.2% were male. At initiation of first-line cART, 23 (62.2%) were WHO stage III, mean CD4 cell count was 170.6 (range: 16-496) cells/µL and median (IQR) HIV-1 viral load was 30 220 (7963-82 598) copies/mL. Most common second-line cART regimens at the time of failure were tenofovir disoproxil fumarate (TDF)-lamivudine (3TC)-ritonavir-boosted atazanavir (ATV/r) (19/37, 51.4%) and zidovudine (ZDV)-3TC-ATV/r (9/37, 24.3%). Genotypic HIV-1 resistance testing was successful in 35 (94.6%) participants. We found at least one resistance mutation in 80% of patients and 40% carried a protease inhibitor (PI)-associated mutation. Most common mutations were M184V (57.1%), Y188C (25.7%), M46I/L (25.7%) and V82A/M (25.7%). High-level resistance against the PI ATV (10/35, 28.6%) and lopinavir (LPV) (5/35, 14.3%) was reported. As expected, no resistance mutations conferring integrase inhibitor resistance were detected.
CONCLUSIONS: We found a high prevalence of resistance mutations, also against PIs (40%), as the national standard second-line cART components. Resistance testing before switching to second- or third-line cART is warranted.
Medienart: |
E-Artikel |
---|
Erscheinungsjahr: |
2022 |
---|---|
Erschienen: |
2022 |
Enthalten in: |
Zur Gesamtaufnahme - volume:23 |
---|---|
Enthalten in: |
HIV medicine - 23(2022), 2 vom: 13. Feb., Seite 159-168 |
Sprache: |
Englisch |
---|
Beteiligte Personen: |
Tufa, Tafese Beyene [VerfasserIn] |
---|
Links: |
---|
Anmerkungen: |
Date Completed 14.03.2022 Date Revised 14.03.2022 published: Print-Electronic Citation Status MEDLINE |
---|
doi: |
10.1111/hiv.13176 |
---|
funding: |
|
---|---|
Förderinstitution / Projekttitel: |
|
PPN (Katalog-ID): |
NLM33163547X |
---|
LEADER | 01000naa a22002652 4500 | ||
---|---|---|---|
001 | NLM33163547X | ||
003 | DE-627 | ||
005 | 20231225213908.0 | ||
007 | cr uuu---uuuuu | ||
008 | 231225s2022 xx |||||o 00| ||eng c | ||
024 | 7 | |a 10.1111/hiv.13176 |2 doi | |
028 | 5 | 2 | |a pubmed24n1105.xml |
035 | |a (DE-627)NLM33163547X | ||
035 | |a (NLM)34622550 | ||
040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
041 | |a eng | ||
100 | 1 | |a Tufa, Tafese Beyene |e verfasserin |4 aut | |
245 | 1 | 0 | |a Characterization of HIV-1 drug resistance among patients with failure of second-line combined antiretroviral therapy in central Ethiopia |
264 | 1 | |c 2022 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a ƒaComputermedien |b c |2 rdamedia | ||
338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
500 | |a Date Completed 14.03.2022 | ||
500 | |a Date Revised 14.03.2022 | ||
500 | |a published: Print-Electronic | ||
500 | |a Citation Status MEDLINE | ||
520 | |a © 2021 The Authors. HIV Medicine published by John Wiley & Sons Ltd on behalf of British HIV Association. | ||
520 | |a BACKGROUND: As a consequence of the improved availability of combined antiretroviral therapy (cART) in resource-limited countries, an emergence of HIV drug resistance (HIVDR) has been observed. We assessed the prevalence and spectrum of HIVDR in patients with failure of second-line cART at two HIV clinics in central Ethiopia | ||
520 | |a METHODS: HIV drug resistance was analysed in HIV-1-infected patients with virological failure of second-line cART using the geno2pheno application | ||
520 | |a RESULTS: Among 714 patients receiving second-line cART, 44 (6.2%) fulfilled the criteria for treatment failure and 37 were eligible for study inclusion. Median age was 42 years [interquartile range (IQR): 20-45] and 62.2% were male. At initiation of first-line cART, 23 (62.2%) were WHO stage III, mean CD4 cell count was 170.6 (range: 16-496) cells/µL and median (IQR) HIV-1 viral load was 30 220 (7963-82 598) copies/mL. Most common second-line cART regimens at the time of failure were tenofovir disoproxil fumarate (TDF)-lamivudine (3TC)-ritonavir-boosted atazanavir (ATV/r) (19/37, 51.4%) and zidovudine (ZDV)-3TC-ATV/r (9/37, 24.3%). Genotypic HIV-1 resistance testing was successful in 35 (94.6%) participants. We found at least one resistance mutation in 80% of patients and 40% carried a protease inhibitor (PI)-associated mutation. Most common mutations were M184V (57.1%), Y188C (25.7%), M46I/L (25.7%) and V82A/M (25.7%). High-level resistance against the PI ATV (10/35, 28.6%) and lopinavir (LPV) (5/35, 14.3%) was reported. As expected, no resistance mutations conferring integrase inhibitor resistance were detected | ||
520 | |a CONCLUSIONS: We found a high prevalence of resistance mutations, also against PIs (40%), as the national standard second-line cART components. Resistance testing before switching to second- or third-line cART is warranted | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a Research Support, Non-U.S. Gov't | |
650 | 4 | |a Africa | |
650 | 4 | |a HIV | |
650 | 4 | |a cART | |
650 | 4 | |a eastern Africa | |
650 | 4 | |a genotypic resistance testing | |
650 | 4 | |a resistance mutations | |
650 | 4 | |a second-line cART | |
650 | 7 | |a Anti-HIV Agents |2 NLM | |
650 | 7 | |a Lopinavir |2 NLM | |
650 | 7 | |a 2494G1JF75 |2 NLM | |
650 | 7 | |a Lamivudine |2 NLM | |
650 | 7 | |a 2T8Q726O95 |2 NLM | |
650 | 7 | |a Ritonavir |2 NLM | |
650 | 7 | |a O3J8G9O825 |2 NLM | |
700 | 1 | |a Fuchs, Andre |e verfasserin |4 aut | |
700 | 1 | |a Orth, Hans Martin |e verfasserin |4 aut | |
700 | 1 | |a Lübke, Nadine |e verfasserin |4 aut | |
700 | 1 | |a Knops, Elena |e verfasserin |4 aut | |
700 | 1 | |a Heger, Eva |e verfasserin |4 aut | |
700 | 1 | |a Jarso, Godana |e verfasserin |4 aut | |
700 | 1 | |a Hurissa, Zewdu |e verfasserin |4 aut | |
700 | 1 | |a Eggers, Yannik |e verfasserin |4 aut | |
700 | 1 | |a Häussinger, Dieter |e verfasserin |4 aut | |
700 | 1 | |a Luedde, Tom |e verfasserin |4 aut | |
700 | 1 | |a Jensen, Björn-Erik Ole |e verfasserin |4 aut | |
700 | 1 | |a Kaiser, Rolf |e verfasserin |4 aut | |
700 | 1 | |a Feldt, Torsten |e verfasserin |4 aut | |
773 | 0 | 8 | |i Enthalten in |t HIV medicine |d 1999 |g 23(2022), 2 vom: 13. Feb., Seite 159-168 |w (DE-627)NLM116282541 |x 1468-1293 |7 nnns |
773 | 1 | 8 | |g volume:23 |g year:2022 |g number:2 |g day:13 |g month:02 |g pages:159-168 |
856 | 4 | 0 | |u http://dx.doi.org/10.1111/hiv.13176 |3 Volltext |
912 | |a GBV_USEFLAG_A | ||
912 | |a GBV_NLM | ||
951 | |a AR | ||
952 | |d 23 |j 2022 |e 2 |b 13 |c 02 |h 159-168 |