SARS-CoV-2 rapid antigen test in comparison to RT-PCR targeting different genes : A real-life evaluation among unselected patients in a regional hospital of Italy
© 2021 The Authors. Journal of Medical Virology published by Wiley Periodicals LLC..
We assessed the performance of the Panbio rapid antigen detection (RAD) test for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection and we compared it with the routine reverse transcriptase-polymerase chain reaction (RT-PCR)-based molecular test in a population of 4167 unselected patients admitted to IRCCS Sacro Cuore Don Calabria Hospital. Analysis stratified by cycling threshold (Ct ) value of SARS-CoV-2 gene targets indicated that antigen (Ag)-positive Ct values were significantly lower compared to Ag-negative values (p < 0.0001). Overall, we found discordance in 140, tested negative by RAD and positive by RT-PCR, and in 4 resulted positive by RAD and negative by RT-PCR. RAD test achieved a sensitivity and specificity of 66.82% and 99.89%, respectively. The positive predictive value was shown to be 97.87% while the negative predictive value was shown to be 97.62%. In our context, the RAD test showed a reliable diagnostic response in subjects that displayed high Ct values, corresponding to high viral load, while low ability was displayed to identify positive cases with medium-low Ct values, thus presenting low viral load and where confirmatory RT-PCR was needed. Our finding supports the use of the RAD test in real-life settings where a high volume of swabs is being processed but with caution when interpreting a positive test result in a low prevalence setting.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:94 |
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| Enthalten in: |
Journal of medical virology - 94(2022), 3 vom: 07. März, Seite 1190-1195 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Treggiari, Davide [VerfasserIn] |
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| Links: |
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| Themen: |
Antigens, Viral |
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| Anmerkungen: |
Date Completed 03.06.2022 Date Revised 05.10.2022 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1002/jmv.27378 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a © 2021 The Authors. Journal of Medical Virology published by Wiley Periodicals LLC. | ||
| 520 | |a We assessed the performance of the Panbio rapid antigen detection (RAD) test for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection and we compared it with the routine reverse transcriptase-polymerase chain reaction (RT-PCR)-based molecular test in a population of 4167 unselected patients admitted to IRCCS Sacro Cuore Don Calabria Hospital. Analysis stratified by cycling threshold (Ct ) value of SARS-CoV-2 gene targets indicated that antigen (Ag)-positive Ct values were significantly lower compared to Ag-negative values (p < 0.0001). Overall, we found discordance in 140, tested negative by RAD and positive by RT-PCR, and in 4 resulted positive by RAD and negative by RT-PCR. RAD test achieved a sensitivity and specificity of 66.82% and 99.89%, respectively. The positive predictive value was shown to be 97.87% while the negative predictive value was shown to be 97.62%. In our context, the RAD test showed a reliable diagnostic response in subjects that displayed high Ct values, corresponding to high viral load, while low ability was displayed to identify positive cases with medium-low Ct values, thus presenting low viral load and where confirmatory RT-PCR was needed. Our finding supports the use of the RAD test in real-life settings where a high volume of swabs is being processed but with caution when interpreting a positive test result in a low prevalence setting | ||
| 650 | 4 | |a Journal Article | |
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| 700 | 1 | |a Ragusa, Andrea |e verfasserin |4 aut | |
| 700 | 1 | |a Orza, Pierantonio |e verfasserin |4 aut | |
| 700 | 1 | |a Pajola, Barbara |e verfasserin |4 aut | |
| 700 | 1 | |a Piccoli, Donatella |e verfasserin |4 aut | |
| 700 | 1 | |a Conti, Antonio |e verfasserin |4 aut | |
| 700 | 1 | |a Lorenzi, Carlo |e verfasserin |4 aut | |
| 700 | 1 | |a Serafini, Valentina |e verfasserin |4 aut | |
| 700 | 1 | |a Boni, Marco |e verfasserin |4 aut | |
| 700 | 1 | |a Perandin, Francesca |e verfasserin |4 aut | |
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