Metagenomic analysis of community, enzymes and metabolic pathways during corn straw fermentation with rumen microorganisms for volatile fatty acid production
Copyright © 2021 Elsevier Ltd. All rights reserved..
Anaerobic fermentation of corn straw with rumen microorganisms as inoculum to produce volatile fatty acids (VFAs) is important for biomass valorization. In this study, dynamic variation in bacterial and fungal community composition, carbohydrate-active enzymes (CAZymes) and key functional genes related with VFA production was explored via metagenomic sequencing. Rumen microorganisms efficiently hydrolyzed and acidified corn straw, and VFA concentration reached 8.99 g/L in 72 h. Bacterial and fungal community significantly changed, but the core genera kept stable. Low pH and VFA accumulation were the main factors affecting bacterial and fungal communities. The positive correlations between bacteria were more complex than those between fungi. Most CAZyme abundance significantly decreased after 72 h fermentation, and functional gene abundance participating in VFA generation also decreased. This study provided new insights into dynamic variation of bacteria and fungi during anaerobic ruminal fermentation in vitro, promoting the application of rumen microorganisms in practice.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:342 |
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| Enthalten in: |
Bioresource technology - 342(2021) vom: 01. Dez., Seite 126004 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Liang, Jinsong [VerfasserIn] |
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| Links: |
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| Themen: |
Anaerobic fermentation |
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| Anmerkungen: |
Date Completed 03.12.2021 Date Revised 14.12.2021 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.biortech.2021.126004 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM331246562 |
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| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2021 Elsevier Ltd. All rights reserved. | ||
| 520 | |a Anaerobic fermentation of corn straw with rumen microorganisms as inoculum to produce volatile fatty acids (VFAs) is important for biomass valorization. In this study, dynamic variation in bacterial and fungal community composition, carbohydrate-active enzymes (CAZymes) and key functional genes related with VFA production was explored via metagenomic sequencing. Rumen microorganisms efficiently hydrolyzed and acidified corn straw, and VFA concentration reached 8.99 g/L in 72 h. Bacterial and fungal community significantly changed, but the core genera kept stable. Low pH and VFA accumulation were the main factors affecting bacterial and fungal communities. The positive correlations between bacteria were more complex than those between fungi. Most CAZyme abundance significantly decreased after 72 h fermentation, and functional gene abundance participating in VFA generation also decreased. This study provided new insights into dynamic variation of bacteria and fungi during anaerobic ruminal fermentation in vitro, promoting the application of rumen microorganisms in practice | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Anaerobic fermentation | |
| 650 | 4 | |a Carbohydrate-active enzymes | |
| 650 | 4 | |a Co-occurrence network | |
| 650 | 4 | |a Metabolic pathways | |
| 650 | 4 | |a Rumen microorganisms | |
| 650 | 7 | |a Fatty Acids, Volatile |2 NLM | |
| 700 | 1 | |a Fang, Wei |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Qingyan |e verfasserin |4 aut | |
| 700 | 1 | |a Zubair, Muhammad |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Guangming |e verfasserin |4 aut | |
| 700 | 1 | |a Ma, Weifang |e verfasserin |4 aut | |
| 700 | 1 | |a Cai, Yajing |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Panyue |e verfasserin |4 aut | |
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