Digital Microfluidic Hemagglutination Assays for Blood Typing, Donor Compatibility Testing, and Hematocrit Analysis
© American Association for Clinical Chemistry 2021. All rights reserved. For permissions, please email: journals.permissionsoup.com..
BACKGROUND: Blood typing, donor compatibility testing, and hematocrit analysis are common tests that are important in many clinical applications, including those found in high-stakes settings such as the trauma center. These tests are typically performed in centralized laboratories with sample batching; the minutes that are lost in this mode can lead to adverse outcomes, especially for critical-care patients. As a step toward providing rapid results at the bedside, we developed a point-of-care hemagglutination system relying on digital microfluidics (DMF) and a unique, automated readout tool, droplet agglutination assessment using digital microfluidics (DAAD).
METHODS: ABO and Rhesus blood grouping, donor crossmatching, and hematocrit assays were developed on a portable DMF platform that allowed for automated sample processing. The result of each assay could be determined by eye or automatically with the DAAD imaging tool.
RESULTS: DMF-DAAD was applied to 109 samples collected from different sources (including commercial samples, pinpricks from volunteers, and a hospital blood bank), with perfect fidelity to gold-standard results. Some of these tests were carried out by a nonexpert in a hospital trauma center. Proof-of-concept results were also collected from smaller sample sets for donor compatibility testing and hematocrit analysis.
CONCLUSION: DMF-DAAD shows promise for delivering rapid, reliable results in a format well suited for a trauma center and other settings where every minute counts.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:67 |
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| Enthalten in: |
Clinical chemistry - 67(2021), 12 vom: 26. Nov., Seite 1699-1708 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Sklavounos, Alexandros A [VerfasserIn] |
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| Links: |
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| Themen: |
Instrumentation |
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| Anmerkungen: |
Date Completed 13.04.2022 Date Revised 13.04.2022 published: Print Citation Status MEDLINE |
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| doi: |
10.1093/clinchem/hvab180 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM331222930 |
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| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © American Association for Clinical Chemistry 2021. All rights reserved. For permissions, please email: journals.permissionsoup.com. | ||
| 520 | |a BACKGROUND: Blood typing, donor compatibility testing, and hematocrit analysis are common tests that are important in many clinical applications, including those found in high-stakes settings such as the trauma center. These tests are typically performed in centralized laboratories with sample batching; the minutes that are lost in this mode can lead to adverse outcomes, especially for critical-care patients. As a step toward providing rapid results at the bedside, we developed a point-of-care hemagglutination system relying on digital microfluidics (DMF) and a unique, automated readout tool, droplet agglutination assessment using digital microfluidics (DAAD) | ||
| 520 | |a METHODS: ABO and Rhesus blood grouping, donor crossmatching, and hematocrit assays were developed on a portable DMF platform that allowed for automated sample processing. The result of each assay could be determined by eye or automatically with the DAAD imaging tool | ||
| 520 | |a RESULTS: DMF-DAAD was applied to 109 samples collected from different sources (including commercial samples, pinpricks from volunteers, and a hospital blood bank), with perfect fidelity to gold-standard results. Some of these tests were carried out by a nonexpert in a hospital trauma center. Proof-of-concept results were also collected from smaller sample sets for donor compatibility testing and hematocrit analysis | ||
| 520 | |a CONCLUSION: DMF-DAAD shows promise for delivering rapid, reliable results in a format well suited for a trauma center and other settings where every minute counts | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a instrumentation | |
| 650 | 4 | |a laboratory methods and tools | |
| 650 | 4 | |a point-of-care testing systems | |
| 700 | 1 | |a Lamanna, Julian |e verfasserin |4 aut | |
| 700 | 1 | |a Modi, Dimpy |e verfasserin |4 aut | |
| 700 | 1 | |a Gupta, Sidharth |e verfasserin |4 aut | |
| 700 | 1 | |a Mariakakis, Alex |e verfasserin |4 aut | |
| 700 | 1 | |a Callum, Jeannie |e verfasserin |4 aut | |
| 700 | 1 | |a Wheeler, Aaron R |e verfasserin |4 aut | |
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