Mast Cell Distribution in Human Carotid Atherosclerotic Plaque Differs Significantly by Histological Segment
Copyright © 2021. Published by Elsevier B.V..
OBJECTIVE: Mast cells (MCs) are important contributors to atherosclerotic plaque progression. For prospective studies on mast cell contributions to plaque instability, the distribution of intraplaque MCs needs to be elucidated. Plaque stability is generally histologically assessed by dividing the plaque specimen into segments to be scored on an ordinal scale. However, owing to competitive use, studies may have to deviate to adjacent segments, yet intersegment differences of plaque characteristics, especially MCs, are largely unknown. Therefore, the hypothesis that there is no segment to segment difference in MC distribution between atherosclerotic plaque segments was tested, and intersegment associations between MCs and other plaque characteristics was investigated.
METHODS: Twenty-six carotid atherosclerotic plaques from patients undergoing carotid endarterectomy included in the Athero-Express Biobank were analysed. The plaque was divided in 5 mm segments, differentiating between the culprit lesion (segment 0), adjacent segments (-1/+1) and more distant segments (-2/+2) for the presence of MCs. The associations between the intersegment distribution of MCs and smooth muscle cells, macrophage content, and microvessel density in the culprit lesion were studied.
RESULTS: A statistically significant difference in MCs/mm2 between the different plaque segments (p < .001) was found, with a median of 2.79 (interquartile range [IQR] 1.63 - 7.10) for the culprit lesion, 1.34 (IQR 0.26 - 4.45) for the adjacent segment, and 0.62 (0.14 - 2.07) for the more distant segment. Post hoc analyses showed that intersegment differences were due to differences in MCs/mm2 between the culprit and adjacent segment (p = .037) and between the culprit lesion and the more distant segment (p < .001). MCs/mm2 in multiple different segments were positively correlated with microvessel density and macrophage content in the culprit lesion.
CONCLUSION: MC numbers reveal significant intersegment differences in human carotid plaques. Future histological studies on MCs should use a standardised segment for plaque characterisation as plaque segments cannot be used interchangeably for histological MC analyses.
| Errataetall: |
CommentIn: Eur J Vasc Endovasc Surg. 2021 Nov;62(5):816. - PMID 34511316 |
|---|---|
| Medienart: |
E-Artikel |
| Erscheinungsjahr: |
2021 |
|---|---|
| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:62 |
|---|---|
| Enthalten in: |
European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery - 62(2021), 5 vom: 28. Nov., Seite 808-815 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Mekke, Joost M [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Atherosclerosis |
|---|
| Anmerkungen: |
Date Completed 22.12.2021 Date Revised 22.12.2021 published: Print-Electronic CommentIn: Eur J Vasc Endovasc Surg. 2021 Nov;62(5):816. - PMID 34511316 Citation Status MEDLINE |
|---|
| doi: |
10.1016/j.ejvs.2021.07.008 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM330730894 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM330730894 | ||
| 003 | DE-627 | ||
| 005 | 20231225211919.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2021 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.ejvs.2021.07.008 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1102.xml |
| 035 | |a (DE-627)NLM330730894 | ||
| 035 | |a (NLM)34531119 | ||
| 035 | |a (PII)S1078-5884(21)00600-6 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Mekke, Joost M |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Mast Cell Distribution in Human Carotid Atherosclerotic Plaque Differs Significantly by Histological Segment |
| 264 | 1 | |c 2021 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 22.12.2021 | ||
| 500 | |a Date Revised 22.12.2021 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a CommentIn: Eur J Vasc Endovasc Surg. 2021 Nov;62(5):816. - PMID 34511316 | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2021. Published by Elsevier B.V. | ||
| 520 | |a OBJECTIVE: Mast cells (MCs) are important contributors to atherosclerotic plaque progression. For prospective studies on mast cell contributions to plaque instability, the distribution of intraplaque MCs needs to be elucidated. Plaque stability is generally histologically assessed by dividing the plaque specimen into segments to be scored on an ordinal scale. However, owing to competitive use, studies may have to deviate to adjacent segments, yet intersegment differences of plaque characteristics, especially MCs, are largely unknown. Therefore, the hypothesis that there is no segment to segment difference in MC distribution between atherosclerotic plaque segments was tested, and intersegment associations between MCs and other plaque characteristics was investigated | ||
| 520 | |a METHODS: Twenty-six carotid atherosclerotic plaques from patients undergoing carotid endarterectomy included in the Athero-Express Biobank were analysed. The plaque was divided in 5 mm segments, differentiating between the culprit lesion (segment 0), adjacent segments (-1/+1) and more distant segments (-2/+2) for the presence of MCs. The associations between the intersegment distribution of MCs and smooth muscle cells, macrophage content, and microvessel density in the culprit lesion were studied | ||
| 520 | |a RESULTS: A statistically significant difference in MCs/mm2 between the different plaque segments (p < .001) was found, with a median of 2.79 (interquartile range [IQR] 1.63 - 7.10) for the culprit lesion, 1.34 (IQR 0.26 - 4.45) for the adjacent segment, and 0.62 (0.14 - 2.07) for the more distant segment. Post hoc analyses showed that intersegment differences were due to differences in MCs/mm2 between the culprit and adjacent segment (p = .037) and between the culprit lesion and the more distant segment (p < .001). MCs/mm2 in multiple different segments were positively correlated with microvessel density and macrophage content in the culprit lesion | ||
| 520 | |a CONCLUSION: MC numbers reveal significant intersegment differences in human carotid plaques. Future histological studies on MCs should use a standardised segment for plaque characterisation as plaque segments cannot be used interchangeably for histological MC analyses | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Observational Study | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Atherosclerosis | |
| 650 | 4 | |a Carotid Endarterectomy | |
| 650 | 4 | |a Carotid arteries | |
| 650 | 4 | |a Mast cells | |
| 650 | 4 | |a Plaque composition | |
| 700 | 1 | |a Egberts, Daan H J |e verfasserin |4 aut | |
| 700 | 1 | |a Waissi, Farahnaz |e verfasserin |4 aut | |
| 700 | 1 | |a Timmerman, Nathalie |e verfasserin |4 aut | |
| 700 | 1 | |a Bot, Ilze |e verfasserin |4 aut | |
| 700 | 1 | |a Kuiper, Johan |e verfasserin |4 aut | |
| 700 | 1 | |a Pasterkamp, Gerard |e verfasserin |4 aut | |
| 700 | 1 | |a de Borst, Gert J |e verfasserin |4 aut | |
| 700 | 1 | |a de Kleijn, Dominique P V |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery |d 1998 |g 62(2021), 5 vom: 28. Nov., Seite 808-815 |w (DE-627)NLM074658220 |x 1532-2165 |7 nnns |
| 773 | 1 | 8 | |g volume:62 |g year:2021 |g number:5 |g day:28 |g month:11 |g pages:808-815 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.ejvs.2021.07.008 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 62 |j 2021 |e 5 |b 28 |c 11 |h 808-815 | ||