Determination of Sulphasalazine and Its Related Compounds by Simple, Smart, Validated, Green Spectrophotometric Methods
© AOAC INTERNATIONAL 2021. All rights reserved. For permissions, please email: journals.permissionsoup.com..
BACKGROUND: Sulphasalazine (SZ) is a prodrug. Its active metabolite (mesalazine, MZ), which is also available in pharmaceutical formulations, and the major active metabolite of MZ (N-acetyl-5-aminosalicylic acid, AS) are commonly used for the treatment of inflammatory bowel diseases.
OBJECTIVE: Two accurate, precise, sensitive, and specific spectrophotometric methods were developed and validated for determination of the studied components.
METHODS: The first method is a modified ratio difference spectrophotometric method. In this method, SZ was determined by measuring the peak area from 410-500 nm, while MZ and AS were determined by measuring the difference of the selected amplitude values. The second method is a mean centering of ratio spectra spectrophotometric method.
RESULTS: The developed methods were linear over the concentration ranges of 2-35, 2-30 and 1-25 µg/mL for SZ, MZ and AS, respectively.
CONCLUSION: The developed methods were validated according to International Conference on Harmonization guidelines. They were successfully applied for determination of studied analytes. A greenness assessment was undertaken using three different tools.
HIGHLIGHTS: Spectrophotometric methods were developed for determination of SZ and its related compounds for the first time. They were designated to be green and eco-friendly and their greenness profiles were evaluated using green solvents to keep the environment clean.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2022 |
|---|---|
| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:105 |
|---|---|
| Enthalten in: |
Journal of AOAC International - 105(2022), 2 vom: 15. März, Seite 352-361 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Abdelwhab, Nada S [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
|---|
| Anmerkungen: |
Date Completed 17.03.2022 Date Revised 17.03.2022 published: Print Citation Status MEDLINE |
|---|
| doi: |
10.1093/jaoacint/qsab103 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM330619039 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM330619039 | ||
| 003 | DE-627 | ||
| 005 | 20231225211700.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2022 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1093/jaoacint/qsab103 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1102.xml |
| 035 | |a (DE-627)NLM330619039 | ||
| 035 | |a (NLM)34519787 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Abdelwhab, Nada S |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Determination of Sulphasalazine and Its Related Compounds by Simple, Smart, Validated, Green Spectrophotometric Methods |
| 264 | 1 | |c 2022 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 17.03.2022 | ||
| 500 | |a Date Revised 17.03.2022 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © AOAC INTERNATIONAL 2021. All rights reserved. For permissions, please email: journals.permissionsoup.com. | ||
| 520 | |a BACKGROUND: Sulphasalazine (SZ) is a prodrug. Its active metabolite (mesalazine, MZ), which is also available in pharmaceutical formulations, and the major active metabolite of MZ (N-acetyl-5-aminosalicylic acid, AS) are commonly used for the treatment of inflammatory bowel diseases | ||
| 520 | |a OBJECTIVE: Two accurate, precise, sensitive, and specific spectrophotometric methods were developed and validated for determination of the studied components | ||
| 520 | |a METHODS: The first method is a modified ratio difference spectrophotometric method. In this method, SZ was determined by measuring the peak area from 410-500 nm, while MZ and AS were determined by measuring the difference of the selected amplitude values. The second method is a mean centering of ratio spectra spectrophotometric method | ||
| 520 | |a RESULTS: The developed methods were linear over the concentration ranges of 2-35, 2-30 and 1-25 µg/mL for SZ, MZ and AS, respectively | ||
| 520 | |a CONCLUSION: The developed methods were validated according to International Conference on Harmonization guidelines. They were successfully applied for determination of studied analytes. A greenness assessment was undertaken using three different tools | ||
| 520 | |a HIGHLIGHTS: Spectrophotometric methods were developed for determination of SZ and its related compounds for the first time. They were designated to be green and eco-friendly and their greenness profiles were evaluated using green solvents to keep the environment clean | ||
| 650 | 4 | |a Journal Article | |
| 650 | 7 | |a Sulfasalazine |2 NLM | |
| 650 | 7 | |a 3XC8GUZ6CB |2 NLM | |
| 700 | 1 | |a Habib, Neven M |e verfasserin |4 aut | |
| 700 | 1 | |a Abdelrahman, Maha M |e verfasserin |4 aut | |
| 700 | 1 | |a Mahmoud, Hamada M |e verfasserin |4 aut | |
| 700 | 1 | |a Emam, Aml A |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Journal of AOAC International |d 1993 |g 105(2022), 2 vom: 15. März, Seite 352-361 |w (DE-627)NLM074913743 |x 1944-7922 |7 nnns |
| 773 | 1 | 8 | |g volume:105 |g year:2022 |g number:2 |g day:15 |g month:03 |g pages:352-361 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1093/jaoacint/qsab103 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 105 |j 2022 |e 2 |b 15 |c 03 |h 352-361 | ||