MiR-224-5p Targeting OCLN Promotes the Proliferation, Migration, and Invasion of Clear Cell Renal Cell Carcinoma Cells
© 2021 S. Karger AG, Basel..
A number of studies reported that miR-224-5p is involved in a variety of cancer-related cellular processes, yet its physiological role in clear cell renal cell carcinoma (ccRCC) remains unclear. In order to clarify the function of miR-224-5p in ccRCC, real-time quantitative-PCR was conducted to compare the expression of miR-224-5p in human normal renal tubular epithelial cell lines and ccRCC cell lines first, and a strikingly upregulated expression was observed in ccRCC cell lines. Inhibition of miR-224-5p expression by microRNA inhibitors could inhibit the proliferation, migration, and invasion of ccRCC cells. Besides, it was validated by dual-luciferase assay in which miR-224-5p directly targeted OCLN gene. The expression of OCLN was downregulated in ccRCC cells, and overexpression of miR-224-5p could inhibit the mRNA and protein expression levels of OCLN. Overexpression of OCLN could reduce the proliferation, migration, and invasion of ccRCC cells, while overexpressed miR-224-5p could partially reverse that inhibitory effect. Therefore, the promotive effect of miR-224-5p on the proliferation, invasion, and migration of ccRCC cell lines was at least partly due to the inhibition of OCLN expression. These findings highlighted the important function of miR-224-5p, which was promoting cell proliferation, migration, and invasion by downregulating OCLN, in the pathogenesis of ccRCC, and provided a potential treatment strategy.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2022 |
|---|---|
| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:106 |
|---|---|
| Enthalten in: |
Urologia internationalis - 106(2022), 11 vom: 31., Seite 1185-1194 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Liu, Yifei [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Clear cell renal cell carcinoma |
|---|
| Anmerkungen: |
Date Completed 10.11.2022 Date Revised 10.11.2022 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1159/000515981 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM330574140 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM330574140 | ||
| 003 | DE-627 | ||
| 005 | 20231225211557.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2022 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1159/000515981 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1101.xml |
| 035 | |a (DE-627)NLM330574140 | ||
| 035 | |a (NLM)34515239 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Liu, Yifei |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a MiR-224-5p Targeting OCLN Promotes the Proliferation, Migration, and Invasion of Clear Cell Renal Cell Carcinoma Cells |
| 264 | 1 | |c 2022 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 10.11.2022 | ||
| 500 | |a Date Revised 10.11.2022 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2021 S. Karger AG, Basel. | ||
| 520 | |a A number of studies reported that miR-224-5p is involved in a variety of cancer-related cellular processes, yet its physiological role in clear cell renal cell carcinoma (ccRCC) remains unclear. In order to clarify the function of miR-224-5p in ccRCC, real-time quantitative-PCR was conducted to compare the expression of miR-224-5p in human normal renal tubular epithelial cell lines and ccRCC cell lines first, and a strikingly upregulated expression was observed in ccRCC cell lines. Inhibition of miR-224-5p expression by microRNA inhibitors could inhibit the proliferation, migration, and invasion of ccRCC cells. Besides, it was validated by dual-luciferase assay in which miR-224-5p directly targeted OCLN gene. The expression of OCLN was downregulated in ccRCC cells, and overexpression of miR-224-5p could inhibit the mRNA and protein expression levels of OCLN. Overexpression of OCLN could reduce the proliferation, migration, and invasion of ccRCC cells, while overexpressed miR-224-5p could partially reverse that inhibitory effect. Therefore, the promotive effect of miR-224-5p on the proliferation, invasion, and migration of ccRCC cell lines was at least partly due to the inhibition of OCLN expression. These findings highlighted the important function of miR-224-5p, which was promoting cell proliferation, migration, and invasion by downregulating OCLN, in the pathogenesis of ccRCC, and provided a potential treatment strategy | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Clear cell renal cell carcinoma | |
| 650 | 4 | |a Invasion | |
| 650 | 4 | |a Migration | |
| 650 | 4 | |a OCLN | |
| 650 | 4 | |a Proliferation | |
| 650 | 4 | |a miR-224-5p | |
| 650 | 7 | |a MicroRNAs |2 NLM | |
| 650 | 7 | |a OCLN protein, human |2 NLM | |
| 650 | 7 | |a Occludin |2 NLM | |
| 650 | 7 | |a MIRN224 microRNA, human |2 NLM | |
| 700 | 1 | |a Nie, Honglin |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Yubo |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Na |e verfasserin |4 aut | |
| 700 | 1 | |a Han, Miaomiao |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Huancai |e verfasserin |4 aut | |
| 700 | 1 | |a Sun, Dongli |e verfasserin |4 aut | |
| 700 | 1 | |a Wu, Xiaotang |e verfasserin |4 aut | |
| 700 | 1 | |a Xiao, Xiaolong |e verfasserin |4 aut | |
| 700 | 1 | |a Cao, Xiaoning |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Urologia internationalis |d 1955 |g 106(2022), 11 vom: 31., Seite 1185-1194 |w (DE-627)NLM000011215 |x 1423-0399 |7 nnns |
| 773 | 1 | 8 | |g volume:106 |g year:2022 |g number:11 |g day:31 |g pages:1185-1194 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1159/000515981 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 106 |j 2022 |e 11 |b 31 |h 1185-1194 | ||