Prolonged Normothermic Ex Vivo Kidney Perfusion Is Superior to Cold Nonoxygenated and Oxygenated Machine Perfusion for the Preservation of DCD Porcine Kidney Grafts
Copyright © 2021 The Author(s). Transplantation Direct. Published by Wolters Kluwer Health, Inc..
The increased usage of marginal grafts has triggered interest in perfused kidney preservation to minimize graft injury. We used a donation after circulatory death (DCD) porcine kidney autotransplantation model to compare 3 of the most frequently used ex vivo kidney perfusion techniques: nonoxygenated hypothermic machine perfusion (non-oxHMP), oxygenated hypothermic machine perfusion (oxHMP), and normothermic ex vivo kidney perfusion (NEVKP).
METHODS: Following 30 min of warm ischemia, grafts were retrieved and preserved with either 16 h of non-oxHMP, oxHMP, or NEVKP (n = 5 per group). After contralateral nephrectomy, grafts were autotransplanted and animals were followed for 8 d. Kidney function and injury markers were compared between groups.
RESULTS: NEVKP demonstrated a significant reduction in preservation injury compared with either cold preservation method. Grafts preserved by NEVKP showed superior function with lower peak serum creatinine (NEVKP versus non-oxHMP versus oxHMP: 3.66 ± 1.33 mg/dL, 8.82 ± 3.17 mg/dL, and 9.02 ± 5.5 mg/dL) and more rapid recovery. The NEVKP group demonstrated significantly increased creatinine clearance on postoperative day 3 compared with the cold perfused groups. Tubular injury scores on postoperative day 8 were similar in all groups.
CONCLUSIONS: Addition of oxygen during HMP did not reduce preservation injury of DCD kidney grafts. Grafts preserved with prolonged NEVKP demonstrated superior initial graft function compared with grafts preserved with non-oxHMP or oxHMP in a model of pig DCD kidney transplantation.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:7 |
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| Enthalten in: |
Transplantation direct - 7(2021), 10 vom: 24. Okt., Seite e751 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Mazilescu, Laura I [VerfasserIn] |
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| Anmerkungen: |
Date Revised 26.04.2022 published: Electronic-eCollection Citation Status PubMed-not-MEDLINE |
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| doi: |
10.1097/TXD.0000000000001218 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM330562940 |
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| 100 | 1 | |a Mazilescu, Laura I |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Prolonged Normothermic Ex Vivo Kidney Perfusion Is Superior to Cold Nonoxygenated and Oxygenated Machine Perfusion for the Preservation of DCD Porcine Kidney Grafts |
| 264 | 1 | |c 2021 | |
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| 500 | |a Date Revised 26.04.2022 | ||
| 500 | |a published: Electronic-eCollection | ||
| 500 | |a Citation Status PubMed-not-MEDLINE | ||
| 520 | |a Copyright © 2021 The Author(s). Transplantation Direct. Published by Wolters Kluwer Health, Inc. | ||
| 520 | |a The increased usage of marginal grafts has triggered interest in perfused kidney preservation to minimize graft injury. We used a donation after circulatory death (DCD) porcine kidney autotransplantation model to compare 3 of the most frequently used ex vivo kidney perfusion techniques: nonoxygenated hypothermic machine perfusion (non-oxHMP), oxygenated hypothermic machine perfusion (oxHMP), and normothermic ex vivo kidney perfusion (NEVKP) | ||
| 520 | |a METHODS: Following 30 min of warm ischemia, grafts were retrieved and preserved with either 16 h of non-oxHMP, oxHMP, or NEVKP (n = 5 per group). After contralateral nephrectomy, grafts were autotransplanted and animals were followed for 8 d. Kidney function and injury markers were compared between groups | ||
| 520 | |a RESULTS: NEVKP demonstrated a significant reduction in preservation injury compared with either cold preservation method. Grafts preserved by NEVKP showed superior function with lower peak serum creatinine (NEVKP versus non-oxHMP versus oxHMP: 3.66 ± 1.33 mg/dL, 8.82 ± 3.17 mg/dL, and 9.02 ± 5.5 mg/dL) and more rapid recovery. The NEVKP group demonstrated significantly increased creatinine clearance on postoperative day 3 compared with the cold perfused groups. Tubular injury scores on postoperative day 8 were similar in all groups | ||
| 520 | |a CONCLUSIONS: Addition of oxygen during HMP did not reduce preservation injury of DCD kidney grafts. Grafts preserved with prolonged NEVKP demonstrated superior initial graft function compared with grafts preserved with non-oxHMP or oxHMP in a model of pig DCD kidney transplantation | ||
| 650 | 4 | |a Journal Article | |
| 700 | 1 | |a Urbanellis, Peter |e verfasserin |4 aut | |
| 700 | 1 | |a Kaths, Moritz J |e verfasserin |4 aut | |
| 700 | 1 | |a Ganesh, Sujani |e verfasserin |4 aut | |
| 700 | 1 | |a Goto, Toru |e verfasserin |4 aut | |
| 700 | 1 | |a Noguchi, Yuki |e verfasserin |4 aut | |
| 700 | 1 | |a John, Rohan |e verfasserin |4 aut | |
| 700 | 1 | |a Konvalinka, Ana |e verfasserin |4 aut | |
| 700 | 1 | |a Mucsi, Istvan |e verfasserin |4 aut | |
| 700 | 1 | |a Ghanekar, Anand |e verfasserin |4 aut | |
| 700 | 1 | |a Bagli, Darius J |e verfasserin |4 aut | |
| 700 | 1 | |a Turgeon, Julie |e verfasserin |4 aut | |
| 700 | 1 | |a Karakeusian Rimbaud, Annie |e verfasserin |4 aut | |
| 700 | 1 | |a Hébert, Marie-Josée |e verfasserin |4 aut | |
| 700 | 1 | |a Dieudé, Mélanie |e verfasserin |4 aut | |
| 700 | 1 | |a Alleys, Isabelle |e verfasserin |4 aut | |
| 700 | 1 | |a Dore, Etienne |e verfasserin |4 aut | |
| 700 | 1 | |a Boilard, Eric |e verfasserin |4 aut | |
| 700 | 1 | |a Overkleeft, Herman S |e verfasserin |4 aut | |
| 700 | 1 | |a Willems, Lianne I |e verfasserin |4 aut | |
| 700 | 1 | |a Robinson, Lisa A |e verfasserin |4 aut | |
| 700 | 1 | |a Selzner, Markus |e verfasserin |4 aut | |
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