Silk sericin application increases bone morphogenic protein-2/4 expression via a toll-like receptor-mediated pathway
Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved..
Bone morphogenic protein-2/4 (BMP-2/4) is an osteoinductive protein that accelerates osteogenesis when administered to bony defects. Sericin is produced by silkworms, and has a biological activity that differs depending on the degumming method used. Our results indicated that the high molecular weight fraction of silk sericin (MW > 30 kDa) obtained via sonication had a more abundant β-sheet structure than the low molecular weight fraction. Administration of the β-sheet structure silk sericin increased BMP-2/4 expression in a dose-dependent manner in RAW264.7 cells and human monocytes. This sericin increased the expression levels of toll-like receptor (TLR)-2, TLR-3, and TLR-4 in RAW264.7 cells. Application of a TLR-2 antibody or TLR pathway blocker decreased BMP-2/4 expression following sericin administration. In the animal model, the bone volume and BMP-2/4 expression were higher in rats treated with a sericin-incorporated gelatin sponge than in rats treated with a gelatin sponge alone or a sponge-incorporated with denatured sericin. In conclusion, sericin with a more abundant β-sheet structure increased BMP-2/4 expression and bone formation better than sericin with a less abundant β-sheet structure.
Medienart: |
E-Artikel |
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Erscheinungsjahr: |
2021 |
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Erschienen: |
2021 |
Enthalten in: |
Zur Gesamtaufnahme - volume:190 |
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Enthalten in: |
International journal of biological macromolecules - 190(2021) vom: 01. Nov., Seite 607-617 |
Sprache: |
Englisch |
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Beteiligte Personen: |
Jo, You-Young [VerfasserIn] |
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Links: |
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Themen: |
9000-70-8 |
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Anmerkungen: |
Date Completed 16.12.2021 Date Revised 26.02.2024 published: Print-Electronic Citation Status MEDLINE |
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doi: |
10.1016/j.ijbiomac.2021.09.021 |
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funding: |
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Förderinstitution / Projekttitel: |
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PPN (Katalog-ID): |
NLM330509284 |
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520 | |a Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved. | ||
520 | |a Bone morphogenic protein-2/4 (BMP-2/4) is an osteoinductive protein that accelerates osteogenesis when administered to bony defects. Sericin is produced by silkworms, and has a biological activity that differs depending on the degumming method used. Our results indicated that the high molecular weight fraction of silk sericin (MW > 30 kDa) obtained via sonication had a more abundant β-sheet structure than the low molecular weight fraction. Administration of the β-sheet structure silk sericin increased BMP-2/4 expression in a dose-dependent manner in RAW264.7 cells and human monocytes. This sericin increased the expression levels of toll-like receptor (TLR)-2, TLR-3, and TLR-4 in RAW264.7 cells. Application of a TLR-2 antibody or TLR pathway blocker decreased BMP-2/4 expression following sericin administration. In the animal model, the bone volume and BMP-2/4 expression were higher in rats treated with a sericin-incorporated gelatin sponge than in rats treated with a gelatin sponge alone or a sponge-incorporated with denatured sericin. In conclusion, sericin with a more abundant β-sheet structure increased BMP-2/4 expression and bone formation better than sericin with a less abundant β-sheet structure | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a Bone morphogenic protein | |
650 | 4 | |a Osteogenesis | |
650 | 4 | |a Silk sericin | |
650 | 7 | |a Bone Morphogenetic Protein 2 |2 NLM | |
650 | 7 | |a Bone Morphogenetic Protein 4 |2 NLM | |
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650 | 7 | |a Gelatin |2 NLM | |
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700 | 1 | |a Kim, Dae-Won |e verfasserin |4 aut | |
700 | 1 | |a Baek, Kyunghwa |e verfasserin |4 aut | |
700 | 1 | |a Chae, Weon-Sik |e verfasserin |4 aut | |
700 | 1 | |a Kang, Yei-Jin |e verfasserin |4 aut | |
700 | 1 | |a Oh, Ji-Hyeon |e verfasserin |4 aut | |
700 | 1 | |a Kim, Seong-Gon |e verfasserin |4 aut | |
700 | 1 | |a Garagiola, Umberto |e verfasserin |4 aut | |
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