A cellular mechanism underlying the restoration of thermo/photoperiod-sensitive genic male sterility
Copyright © 2021 The Author. Published by Elsevier Inc. All rights reserved..
During anther development, the transformation of the microspore into mature pollen occurs under the protection of first the tetrad wall and later the pollen wall. Mutations in genes involved in this wall transition often lead to microspore rupture and male sterility; some such mutants, such as the reversible male sterile (rvms) mutant, are thermo/photoperiod-sensitive genic male sterile (P/TGMS) lines. Previous studies have shown that slow development is a general mechanism of P/TGMS fertility restoration. In this study, we identified restorer of rvms-2 (res2), which is an allele of QUARTET 3 (QRT3) encoding a polygalacturonase that shows delayed degradation of the tetrad pectin wall. We found that MS188, a tapetum-specific transcription factor essential for pollen wall formation, can activate QRT3 expression for pectin wall degradation, indicating a non-cell-autonomous pathway involved in the regulation of the cell wall transition. Further assays showed that a delay in degradation of the tetrad pectin wall is responsible for the fertility restoration of rvms and other P/TGMS lines, whereas early expression of QRT3 eliminates low temperature restoration of rvms-2 fertility. Taken together, these results suggest a likely cellular mechanism of fertility restoration in P/TGMS lines, that is, slow development during the cell wall transition of P/TGMS microspores may reduce the requirement for their wall protection and thus support their development into functional pollens, leading to restored fertility.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:14 |
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| Enthalten in: |
Molecular plant - 14(2021), 12 vom: 06. Dez., Seite 2104-2114 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Shi, Qiang-Sheng [VerfasserIn] |
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| Links: |
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| Themen: |
Arabidopsis Proteins |
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| Anmerkungen: |
Date Completed 07.02.2022 Date Revised 07.02.2022 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.molp.2021.08.019 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM330074504 |
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| 100 | 1 | |a Shi, Qiang-Sheng |e verfasserin |4 aut | |
| 245 | 1 | 2 | |a A cellular mechanism underlying the restoration of thermo/photoperiod-sensitive genic male sterility |
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| 520 | |a Copyright © 2021 The Author. Published by Elsevier Inc. All rights reserved. | ||
| 520 | |a During anther development, the transformation of the microspore into mature pollen occurs under the protection of first the tetrad wall and later the pollen wall. Mutations in genes involved in this wall transition often lead to microspore rupture and male sterility; some such mutants, such as the reversible male sterile (rvms) mutant, are thermo/photoperiod-sensitive genic male sterile (P/TGMS) lines. Previous studies have shown that slow development is a general mechanism of P/TGMS fertility restoration. In this study, we identified restorer of rvms-2 (res2), which is an allele of QUARTET 3 (QRT3) encoding a polygalacturonase that shows delayed degradation of the tetrad pectin wall. We found that MS188, a tapetum-specific transcription factor essential for pollen wall formation, can activate QRT3 expression for pectin wall degradation, indicating a non-cell-autonomous pathway involved in the regulation of the cell wall transition. Further assays showed that a delay in degradation of the tetrad pectin wall is responsible for the fertility restoration of rvms and other P/TGMS lines, whereas early expression of QRT3 eliminates low temperature restoration of rvms-2 fertility. Taken together, these results suggest a likely cellular mechanism of fertility restoration in P/TGMS lines, that is, slow development during the cell wall transition of P/TGMS microspores may reduce the requirement for their wall protection and thus support their development into functional pollens, leading to restored fertility | ||
| 650 | 4 | |a Journal Article | |
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| 700 | 1 | |a Lou, Yue |e verfasserin |4 aut | |
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| 700 | 1 | |a Wang, Sheng-Hong |e verfasserin |4 aut | |
| 700 | 1 | |a Zhou, Lei |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Jun-Jie |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Xing-Lu |e verfasserin |4 aut | |
| 700 | 1 | |a Xiong, Shuang-Xi |e verfasserin |4 aut | |
| 700 | 1 | |a Han, Yu |e verfasserin |4 aut | |
| 700 | 1 | |a Zhou, Hai-Sheng |e verfasserin |4 aut | |
| 700 | 1 | |a Huang, Xue-Hui |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Shui |e verfasserin |4 aut | |
| 700 | 1 | |a Zhu, Jun |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Zhong-Nan |e verfasserin |4 aut | |
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