Effects of 2,4-dichlorophenoxyacetic acid on the expression of NLRP3 inflammasome and autophagy-related proteins as well as the protective effect of Lycium barbarum polysaccharide in neonatal rats
© 2021 Wiley Periodicals LLC..
The pesticide 2,4-dichlorophenoxyacetic acid (2,4-D) has neurotoxic effects, but its mechanism is not clear. In this study, a 2,4-D (75 mg/kg. b.w) exposure model was established in SD rats with colostrum. Lipopolysaccharide (1 mg/kg b.w) was used as the positive control, and Lycium barbarum polysaccharide (LBP, 50 mg/kg b.w) was used as an intervention factor to explore the neurotoxic effect of 2,4-D and the neuroprotective effect of LBP. Our research results show that 2,4-D causes a decrease in the number of hippocampal CA3 pyramidal cells and pyknosis in nuclei with a triangular or irregular shape and that rats show signs of anxiety or depression. In rat serum, superoxide dismutase, and glutathione peroxidase activity decreased, while malondialdehyde content increased. Protein and mRNA levels of TNFα, IL-6, IL-1β, IL-18, NLRP3, ASC, caspase-1, IL-1β, IL-18, and p62 increased, while those of LC3-II/LC3-I and Beclin-1 decreased in hippocampal tissues. In conclusion, 2,4-D increased the oxidative stress level, induced neuroinflammatory response, and decreased the autophagy level in experimental rats. LBP may have upregulated the autophagy level in the body by inhibiting the activation of the NLRP3 inflammasome, thus playing a neuroprotective role.
Medienart: |
E-Artikel |
---|
Erscheinungsjahr: |
2021 |
---|---|
Erschienen: |
2021 |
Enthalten in: |
Zur Gesamtaufnahme - volume:36 |
---|---|
Enthalten in: |
Environmental toxicology - 36(2021), 12 vom: 04. Dez., Seite 2454-2466 |
Sprache: |
Englisch |
---|
Beteiligte Personen: |
Zhou, Jian [VerfasserIn] |
---|
Links: |
---|
Anmerkungen: |
Date Completed 04.11.2021 Date Revised 04.11.2021 published: Print-Electronic Citation Status MEDLINE |
---|
doi: |
10.1002/tox.23358 |
---|
funding: |
|
---|---|
Förderinstitution / Projekttitel: |
|
PPN (Katalog-ID): |
NLM33006746X |
---|
LEADER | 01000naa a22002652 4500 | ||
---|---|---|---|
001 | NLM33006746X | ||
003 | DE-627 | ||
005 | 20231225210510.0 | ||
007 | cr uuu---uuuuu | ||
008 | 231225s2021 xx |||||o 00| ||eng c | ||
024 | 7 | |a 10.1002/tox.23358 |2 doi | |
028 | 5 | 2 | |a pubmed24n1100.xml |
035 | |a (DE-627)NLM33006746X | ||
035 | |a (NLM)34464015 | ||
040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
041 | |a eng | ||
100 | 1 | |a Zhou, Jian |e verfasserin |4 aut | |
245 | 1 | 0 | |a Effects of 2,4-dichlorophenoxyacetic acid on the expression of NLRP3 inflammasome and autophagy-related proteins as well as the protective effect of Lycium barbarum polysaccharide in neonatal rats |
264 | 1 | |c 2021 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a ƒaComputermedien |b c |2 rdamedia | ||
338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
500 | |a Date Completed 04.11.2021 | ||
500 | |a Date Revised 04.11.2021 | ||
500 | |a published: Print-Electronic | ||
500 | |a Citation Status MEDLINE | ||
520 | |a © 2021 Wiley Periodicals LLC. | ||
520 | |a The pesticide 2,4-dichlorophenoxyacetic acid (2,4-D) has neurotoxic effects, but its mechanism is not clear. In this study, a 2,4-D (75 mg/kg. b.w) exposure model was established in SD rats with colostrum. Lipopolysaccharide (1 mg/kg b.w) was used as the positive control, and Lycium barbarum polysaccharide (LBP, 50 mg/kg b.w) was used as an intervention factor to explore the neurotoxic effect of 2,4-D and the neuroprotective effect of LBP. Our research results show that 2,4-D causes a decrease in the number of hippocampal CA3 pyramidal cells and pyknosis in nuclei with a triangular or irregular shape and that rats show signs of anxiety or depression. In rat serum, superoxide dismutase, and glutathione peroxidase activity decreased, while malondialdehyde content increased. Protein and mRNA levels of TNFα, IL-6, IL-1β, IL-18, NLRP3, ASC, caspase-1, IL-1β, IL-18, and p62 increased, while those of LC3-II/LC3-I and Beclin-1 decreased in hippocampal tissues. In conclusion, 2,4-D increased the oxidative stress level, induced neuroinflammatory response, and decreased the autophagy level in experimental rats. LBP may have upregulated the autophagy level in the body by inhibiting the activation of the NLRP3 inflammasome, thus playing a neuroprotective role | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a 2,4-dichlorophenoxyacetic acid | |
650 | 4 | |a Lycium barbarum polysaccharide | |
650 | 4 | |a NLRP3 inflammasome | |
650 | 4 | |a autophagy | |
650 | 4 | |a neonatal rats | |
650 | 7 | |a Autophagy-Related Proteins |2 NLM | |
650 | 7 | |a Drugs, Chinese Herbal |2 NLM | |
650 | 7 | |a Inflammasomes |2 NLM | |
650 | 7 | |a NLR Family, Pyrin Domain-Containing 3 Protein |2 NLM | |
650 | 7 | |a Nlrp3 protein, rat |2 NLM | |
650 | 7 | |a lycium barbarum polysaccharide |2 NLM | |
650 | 7 | |a 2,4-Dichlorophenoxyacetic Acid |2 NLM | |
650 | 7 | |a 2577AQ9262 |2 NLM | |
700 | 1 | |a Li, Honghui |e verfasserin |4 aut | |
700 | 1 | |a Wang, Faxuan |e verfasserin |4 aut | |
700 | 1 | |a Wang, Hengquan |e verfasserin |4 aut | |
700 | 1 | |a Chai, Ru |e verfasserin |4 aut | |
700 | 1 | |a Li, Jiangping |e verfasserin |4 aut | |
700 | 1 | |a Jia, Leina |e verfasserin |4 aut | |
700 | 1 | |a Wang, Kai |e verfasserin |4 aut | |
700 | 1 | |a Zhang, Pengju |e verfasserin |4 aut | |
700 | 1 | |a Zhu, Lingqin |e verfasserin |4 aut | |
700 | 1 | |a Yang, Huifang |e verfasserin |4 aut | |
773 | 0 | 8 | |i Enthalten in |t Environmental toxicology |d 2001 |g 36(2021), 12 vom: 04. Dez., Seite 2454-2466 |w (DE-627)NLM112466567 |x 1522-7278 |7 nnns |
773 | 1 | 8 | |g volume:36 |g year:2021 |g number:12 |g day:04 |g month:12 |g pages:2454-2466 |
856 | 4 | 0 | |u http://dx.doi.org/10.1002/tox.23358 |3 Volltext |
912 | |a GBV_USEFLAG_A | ||
912 | |a GBV_NLM | ||
951 | |a AR | ||
952 | |d 36 |j 2021 |e 12 |b 04 |c 12 |h 2454-2466 |