Purification, characterization and immunostimulatory activity of a novel exopolysaccharide from Bacillus sp. H5
Copyright © 2021 Elsevier B.V. All rights reserved..
Crude exopolysaccharides from extracellular polymeric substances produced by the marine bacterium Bacillus sp. H5 were fractionated using DEAE-Sepharose FF and Sephadex G-75 chromatography. The high molecular weight fraction (89.0 kD) from the neutral fraction was designated EPS5SH; it contained mannose, glucosamine, glucose, and galactose in a molar ratio of 1.00: 0.02: 0.07: 0.02. Infra-red, gas chromatography-mass spectrometry, electrospray ionisation-tandem mass spectrometry analysis and nuclear magnetic resonance revealed EPS5SH was a mannan with α-(1 → 4)-Manp, α-(1 → 2)-Manp, α-(1 → 4, 6)-Manp and β-terminal-Manp. Preliminary in vitro experiments revealed that EPS5SH significantly upregulated nitric oxide synthesis and release of pro-inflammatory factors in murine macrophage RAW264.7 cells. Western blot experiments verified the immunostimulatory effects of EPS5SH through the modulation of the NF-κB and MAPK signalling pathways. In conclusion, EPS5SH was a novel immunostimulatory mannan.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:189 |
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| Enthalten in: |
International journal of biological macromolecules - 189(2021) vom: 31. Okt., Seite 649-656 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Wei, Maosheng [VerfasserIn] |
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| Links: |
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| Themen: |
Characteristics |
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| Anmerkungen: |
Date Completed 16.12.2021 Date Revised 16.12.2021 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.ijbiomac.2021.08.159 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM329929798 |
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| 100 | 1 | |a Wei, Maosheng |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Purification, characterization and immunostimulatory activity of a novel exopolysaccharide from Bacillus sp. H5 |
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| 500 | |a Date Revised 16.12.2021 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2021 Elsevier B.V. All rights reserved. | ||
| 520 | |a Crude exopolysaccharides from extracellular polymeric substances produced by the marine bacterium Bacillus sp. H5 were fractionated using DEAE-Sepharose FF and Sephadex G-75 chromatography. The high molecular weight fraction (89.0 kD) from the neutral fraction was designated EPS5SH; it contained mannose, glucosamine, glucose, and galactose in a molar ratio of 1.00: 0.02: 0.07: 0.02. Infra-red, gas chromatography-mass spectrometry, electrospray ionisation-tandem mass spectrometry analysis and nuclear magnetic resonance revealed EPS5SH was a mannan with α-(1 → 4)-Manp, α-(1 → 2)-Manp, α-(1 → 4, 6)-Manp and β-terminal-Manp. Preliminary in vitro experiments revealed that EPS5SH significantly upregulated nitric oxide synthesis and release of pro-inflammatory factors in murine macrophage RAW264.7 cells. Western blot experiments verified the immunostimulatory effects of EPS5SH through the modulation of the NF-κB and MAPK signalling pathways. In conclusion, EPS5SH was a novel immunostimulatory mannan | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Characteristics | |
| 650 | 4 | |a Exopolysaccharide | |
| 650 | 4 | |a Immunostimulatory effects | |
| 650 | 7 | |a DNA, Ribosomal |2 NLM | |
| 650 | 7 | |a Immunologic Factors |2 NLM | |
| 650 | 7 | |a Lipopolysaccharides |2 NLM | |
| 650 | 7 | |a Monosaccharides |2 NLM | |
| 650 | 7 | |a NF-kappa B |2 NLM | |
| 650 | 7 | |a Polysaccharides |2 NLM | |
| 650 | 7 | |a Toll-Like Receptor 4 |2 NLM | |
| 700 | 1 | |a Geng, Lihua |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Qingchi |e verfasserin |4 aut | |
| 700 | 1 | |a Yue, Yang |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Jing |e verfasserin |4 aut | |
| 700 | 1 | |a Wu, Ning |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Xiaoqing |e verfasserin |4 aut | |
| 700 | 1 | |a Sun, Chaomin |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Quanbin |e verfasserin |4 aut | |
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