Algal p-coumaric acid induces oxidative stress and siderophore biosynthesis in the bacterial symbiont Phaeobacter inhibens
Copyright © 2021 Elsevier Ltd. All rights reserved..
The marine alpha-proteobacterium Phaeobacter inhibens engages in intermittent symbioses with microalgae. The symbiosis is biphasic and concludes in a parasitic phase, during which the bacteria release algaecidal metabolites in response to algal p-coumaric acid (pCA). The cell-wide effects of pCA on P. inhibens remain unknown. Herein, we report a microarray-based transcriptomic study and find that genes related to the oxidative stress response and secondary metabolism are upregulated most, while those associated with energy production and motility are downregulated in the presence of pCA. Among genes upregulated is a previously unannotated biosynthetic gene cluster and, using a combination of gene deletions and metabolic profiling, we show that it gives rise to an unreported siderophore, roseobactin. The simultaneous production of algaecides and roseobactin in the parasitic phase allows the bacteria to take up any iron that is released from dying algal cells, thereby securing a limited micronutrient.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2022 |
|---|---|
| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:29 |
|---|---|
| Enthalten in: |
Cell chemical biology - 29(2022), 4 vom: 21. Apr., Seite 670-679.e5 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Wang, Rurun [VerfasserIn] |
|---|
| Links: |
|---|
| Anmerkungen: |
Date Completed 26.04.2022 Date Revised 16.07.2022 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1016/j.chembiol.2021.08.002 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM329807579 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM329807579 | ||
| 003 | DE-627 | ||
| 005 | 20231225205925.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2022 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.chembiol.2021.08.002 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1099.xml |
| 035 | |a (DE-627)NLM329807579 | ||
| 035 | |a (NLM)34437838 | ||
| 035 | |a (PII)S2451-9456(21)00364-0 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Wang, Rurun |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Algal p-coumaric acid induces oxidative stress and siderophore biosynthesis in the bacterial symbiont Phaeobacter inhibens |
| 264 | 1 | |c 2022 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 26.04.2022 | ||
| 500 | |a Date Revised 16.07.2022 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2021 Elsevier Ltd. All rights reserved. | ||
| 520 | |a The marine alpha-proteobacterium Phaeobacter inhibens engages in intermittent symbioses with microalgae. The symbiosis is biphasic and concludes in a parasitic phase, during which the bacteria release algaecidal metabolites in response to algal p-coumaric acid (pCA). The cell-wide effects of pCA on P. inhibens remain unknown. Herein, we report a microarray-based transcriptomic study and find that genes related to the oxidative stress response and secondary metabolism are upregulated most, while those associated with energy production and motility are downregulated in the presence of pCA. Among genes upregulated is a previously unannotated biosynthetic gene cluster and, using a combination of gene deletions and metabolic profiling, we show that it gives rise to an unreported siderophore, roseobactin. The simultaneous production of algaecides and roseobactin in the parasitic phase allows the bacteria to take up any iron that is released from dying algal cells, thereby securing a limited micronutrient | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Research Support, N.I.H., Extramural | |
| 650 | 4 | |a Roseobacter | |
| 650 | 4 | |a biosynthesis | |
| 650 | 4 | |a microbial symbiosis | |
| 650 | 4 | |a natural product | |
| 650 | 4 | |a oxidative stress response | |
| 650 | 4 | |a siderophore | |
| 650 | 4 | |a transcriptomics | |
| 650 | 7 | |a Coumaric Acids |2 NLM | |
| 650 | 7 | |a Siderophores |2 NLM | |
| 650 | 7 | |a p-coumaric acid |2 NLM | |
| 650 | 7 | |a IBS9D1EU3J |2 NLM | |
| 700 | 1 | |a Gallant, Étienne |e verfasserin |4 aut | |
| 700 | 1 | |a Wilson, Maxwell Z |e verfasserin |4 aut | |
| 700 | 1 | |a Wu, Yihan |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Anran |e verfasserin |4 aut | |
| 700 | 1 | |a Gitai, Zemer |e verfasserin |4 aut | |
| 700 | 1 | |a Seyedsayamdost, Mohammad R |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Cell chemical biology |d 2016 |g 29(2022), 4 vom: 21. Apr., Seite 670-679.e5 |w (DE-627)NLM257252177 |x 2451-9448 |7 nnns |
| 773 | 1 | 8 | |g volume:29 |g year:2022 |g number:4 |g day:21 |g month:04 |g pages:670-679.e5 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.chembiol.2021.08.002 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 29 |j 2022 |e 4 |b 21 |c 04 |h 670-679.e5 | ||