hsa_circ_0003222 accelerates stemness and progression of non-small cell lung cancer by sponging miR-527
© 2021. The Author(s)..
The relationship between circular RNA (circRNA) and cancer stem cells (CSCs) is uncertain. We have investigated the combined influence of CSCs, circRNA (hsa_circ_0003222), and immune checkpoint inhibitors in NSCLC progression and therapy resistance. We constructed lung CSCs (LCSCs; PC9 and A549). The effects of hsa_circ_0003222 in vitro were determined by cell counting, colony and sphere formation, and Transwell assays. A tumor xenograft model of metastasis and orthotopic model were built for in vivo analysis. We found that hsa_circ_0003222 was highly expressed in NSCLC tissues and LCSCs. Higher levels of hsa_circ_0003222 were associated with the stage, metastasis, and survival rate of patients with NSCLC. Reduced levels of hsa_circ_0003222 decreased tumor cell proliferation, migration, invasion, stemness-like properties, and chemoresistance. The silencing of hsa_circ_0003222 was found to downregulate PHF21B expression and its downstream, β-catenin by relieving the sponging effect of miR-527. Moreover, silencing hsa_circ_0003222 alleviated NSCLC resistance to anti-programmed cell death-ligand 1 (PD-L1)-based therapy in vivo. Our data demonstrate the significant role of hsa_circ_0003222 in NSCLC cell stemness-like properties. The manipulation of circRNAs in combination with anti-PD-L1 therapy may alleviate NSCLC stemness and progression.
Medienart: |
E-Artikel |
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Erscheinungsjahr: |
2021 |
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Erschienen: |
2021 |
Enthalten in: |
Zur Gesamtaufnahme - volume:12 |
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Enthalten in: |
Cell death & disease - 12(2021), 9 vom: 25. Aug., Seite 807 |
Sprache: |
Englisch |
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Beteiligte Personen: |
Li, Changhui [VerfasserIn] |
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Links: |
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Themen: |
Journal Article |
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Anmerkungen: |
Date Completed 28.01.2022 Date Revised 26.02.2024 published: Electronic Citation Status MEDLINE |
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doi: |
10.1038/s41419-021-04095-8 |
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funding: |
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Förderinstitution / Projekttitel: |
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PPN (Katalog-ID): |
NLM329769685 |
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520 | |a The relationship between circular RNA (circRNA) and cancer stem cells (CSCs) is uncertain. We have investigated the combined influence of CSCs, circRNA (hsa_circ_0003222), and immune checkpoint inhibitors in NSCLC progression and therapy resistance. We constructed lung CSCs (LCSCs; PC9 and A549). The effects of hsa_circ_0003222 in vitro were determined by cell counting, colony and sphere formation, and Transwell assays. A tumor xenograft model of metastasis and orthotopic model were built for in vivo analysis. We found that hsa_circ_0003222 was highly expressed in NSCLC tissues and LCSCs. Higher levels of hsa_circ_0003222 were associated with the stage, metastasis, and survival rate of patients with NSCLC. Reduced levels of hsa_circ_0003222 decreased tumor cell proliferation, migration, invasion, stemness-like properties, and chemoresistance. The silencing of hsa_circ_0003222 was found to downregulate PHF21B expression and its downstream, β-catenin by relieving the sponging effect of miR-527. Moreover, silencing hsa_circ_0003222 alleviated NSCLC resistance to anti-programmed cell death-ligand 1 (PD-L1)-based therapy in vivo. Our data demonstrate the significant role of hsa_circ_0003222 in NSCLC cell stemness-like properties. The manipulation of circRNAs in combination with anti-PD-L1 therapy may alleviate NSCLC stemness and progression | ||
650 | 4 | |a Journal Article | |
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700 | 1 | |a Zhang, Jiaqi |e verfasserin |4 aut | |
700 | 1 | |a Yang, Xiaohua |e verfasserin |4 aut | |
700 | 1 | |a Hu, Cheng |e verfasserin |4 aut | |
700 | 1 | |a Chu, Tianqing |e verfasserin |4 aut | |
700 | 1 | |a Zhong, Runbo |e verfasserin |4 aut | |
700 | 1 | |a Shen, Yinchen |e verfasserin |4 aut | |
700 | 1 | |a Hu, Fang |e verfasserin |4 aut | |
700 | 1 | |a Pan, Feng |e verfasserin |4 aut | |
700 | 1 | |a Xu, Jianlin |e verfasserin |4 aut | |
700 | 1 | |a Lu, Jun |e verfasserin |4 aut | |
700 | 1 | |a Zheng, Xiaoxuan |e verfasserin |4 aut | |
700 | 1 | |a Zhang, Hai |e verfasserin |4 aut | |
700 | 1 | |a Nie, Wei |e verfasserin |4 aut | |
700 | 1 | |a Han, Baohui |e verfasserin |4 aut | |
700 | 1 | |a Zhang, Xueyan |e verfasserin |4 aut | |
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