A switchable and signal-amplified aptasensor based on metal organic frameworks as the quencher for turn-on detection of T-2 mycotoxin
© 2021. Springer-Verlag GmbH Germany, part of Springer Nature..
A simple and low-cost fluorescence aptasensor was developed for rapid and sensitive signal amplification detection of T-2 mycotoxin (T-2). Dual-terminal-fluorescein amidite (FAM)-labeled aptamer (D-aptamer) acted as a recognition element and signal indicator. The metal organic frameworks (MOFs) of N, N'-bis(2-hydroxyethyl)dithiooxamidato copper (II) (H2dtoaCu) were as the quencher. The D-aptamer was initially adsorbed to the surface of H2dtoaCu, leading to efficient quenching of the aptasensor. Upon addition of T-2, the D-aptamer underwent a conformation change to form the T-2/T-2 aptamer complex, which induced the signaling probe to be released from the H2dtoaCu surface. Thus, the fluorescence intensity (FL) of the D-aptamer was recovered. Versus the single-terminal-FAM-labeled aptamer (S-aptamer), the D-aptamer showed a lower detection limit of 0.39 ng/mL. The aptasensor was also successfully applied to detect T-2 in corn and wheat samples with good recoveries.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:413 |
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| Enthalten in: |
Analytical and bioanalytical chemistry - 413(2021), 26 vom: 22. Nov., Seite 6595-6603 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Tan, Xinliu [VerfasserIn] |
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| Links: |
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| Themen: |
789U1901C5 |
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| Anmerkungen: |
Date Completed 21.12.2021 Date Revised 21.12.2021 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1007/s00216-021-03625-9 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM329742086 |
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| 245 | 1 | 2 | |a A switchable and signal-amplified aptasensor based on metal organic frameworks as the quencher for turn-on detection of T-2 mycotoxin |
| 264 | 1 | |c 2021 | |
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| 500 | |a Date Revised 21.12.2021 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2021. Springer-Verlag GmbH Germany, part of Springer Nature. | ||
| 520 | |a A simple and low-cost fluorescence aptasensor was developed for rapid and sensitive signal amplification detection of T-2 mycotoxin (T-2). Dual-terminal-fluorescein amidite (FAM)-labeled aptamer (D-aptamer) acted as a recognition element and signal indicator. The metal organic frameworks (MOFs) of N, N'-bis(2-hydroxyethyl)dithiooxamidato copper (II) (H2dtoaCu) were as the quencher. The D-aptamer was initially adsorbed to the surface of H2dtoaCu, leading to efficient quenching of the aptasensor. Upon addition of T-2, the D-aptamer underwent a conformation change to form the T-2/T-2 aptamer complex, which induced the signaling probe to be released from the H2dtoaCu surface. Thus, the fluorescence intensity (FL) of the D-aptamer was recovered. Versus the single-terminal-FAM-labeled aptamer (S-aptamer), the D-aptamer showed a lower detection limit of 0.39 ng/mL. The aptasensor was also successfully applied to detect T-2 in corn and wheat samples with good recoveries | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Aptamer | |
| 650 | 4 | |a Fluorescence | |
| 650 | 4 | |a Metal organic frameworks | |
| 650 | 4 | |a T-2 mycotoxin | |
| 650 | 4 | |a Turn-on | |
| 650 | 7 | |a Aptamers, Nucleotide |2 NLM | |
| 650 | 7 | |a Metal-Organic Frameworks |2 NLM | |
| 650 | 7 | |a Mycotoxins |2 NLM | |
| 650 | 7 | |a Copper |2 NLM | |
| 650 | 7 | |a 789U1901C5 |2 NLM | |
| 700 | 1 | |a Yu, Weidao |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Yuwen |e verfasserin |4 aut | |
| 700 | 1 | |a Song, Ping |e verfasserin |4 aut | |
| 700 | 1 | |a Xu, Qing |e verfasserin |4 aut | |
| 700 | 1 | |a Ming, Dengming |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Yaqiong |e verfasserin |4 aut | |
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