Multilocus sequence typing of Treponema pallidum subsp. pallidum in Barcelona
Aim: To implement the multilocus sequence typing (MLST) methodology in syphilis samples previously characterized by enhanced CDC typing (ECDCT) and macrolide resistance. Materials & methods: MLST was performed on genital ulcer and blood samples by analyzing a region of the tp0136, tp0548 and tp0705loci using Sanger sequencing. Results: Up to 59/85 (69.4%) of genital ulcer and 4/39 (10.3%) of whole blood samples were fully typed. The most frequent profiles were 1.3.1 (56%) and 1.1.1 (11%). All the 1.3.1 samples typed carried the A2058G mutation, responsible for macrolide resistance. MLST and ECDCT showed similar overall typing yields. Conclusion: Several allelic profiles of T. pallidum subsp. pallidum were identified and classified into two major genetic clades in Barcelona. Our results were similar to that described in Europe.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2021 |
|---|---|
| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:16 |
|---|---|
| Enthalten in: |
Future microbiology - 16(2021) vom: 09. Sept., Seite 967-976 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Fernández-Naval, Candela [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Anti-Bacterial Agents |
|---|
| Anmerkungen: |
Date Completed 04.01.2022 Date Revised 04.01.2022 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.2217/fmb-2021-0037 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM329582097 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM329582097 | ||
| 003 | DE-627 | ||
| 005 | 20231225205426.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2021 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.2217/fmb-2021-0037 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1098.xml |
| 035 | |a (DE-627)NLM329582097 | ||
| 035 | |a (NLM)34414771 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Fernández-Naval, Candela |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Multilocus sequence typing of Treponema pallidum subsp. pallidum in Barcelona |
| 264 | 1 | |c 2021 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 04.01.2022 | ||
| 500 | |a Date Revised 04.01.2022 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Aim: To implement the multilocus sequence typing (MLST) methodology in syphilis samples previously characterized by enhanced CDC typing (ECDCT) and macrolide resistance. Materials & methods: MLST was performed on genital ulcer and blood samples by analyzing a region of the tp0136, tp0548 and tp0705loci using Sanger sequencing. Results: Up to 59/85 (69.4%) of genital ulcer and 4/39 (10.3%) of whole blood samples were fully typed. The most frequent profiles were 1.3.1 (56%) and 1.1.1 (11%). All the 1.3.1 samples typed carried the A2058G mutation, responsible for macrolide resistance. MLST and ECDCT showed similar overall typing yields. Conclusion: Several allelic profiles of T. pallidum subsp. pallidum were identified and classified into two major genetic clades in Barcelona. Our results were similar to that described in Europe | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Treponema pallidum | |
| 650 | 4 | |a macrolide resistance | |
| 650 | 4 | |a molecular epidemiology | |
| 650 | 4 | |a molecular typing | |
| 650 | 4 | |a multilocus sequence typing | |
| 650 | 4 | |a phylogeny | |
| 650 | 4 | |a syphilis | |
| 650 | 7 | |a Anti-Bacterial Agents |2 NLM | |
| 650 | 7 | |a Macrolides |2 NLM | |
| 700 | 1 | |a Arando, Maider |e verfasserin |4 aut | |
| 700 | 1 | |a Espasa, Mateu |e verfasserin |4 aut | |
| 700 | 1 | |a Antón, Andrés |e verfasserin |4 aut | |
| 700 | 1 | |a Fernández-Huerta, Miguel |e verfasserin |4 aut | |
| 700 | 1 | |a Silgado, Aroa |e verfasserin |4 aut | |
| 700 | 1 | |a Pinatar, Cristina |e verfasserin |4 aut | |
| 700 | 1 | |a Zarzuela, Francesc |e verfasserin |4 aut | |
| 700 | 1 | |a González-López, Juan José |e verfasserin |4 aut | |
| 700 | 1 | |a Serra-Pladevall, Judit |e verfasserin |4 aut | |
| 700 | 1 | |a Sulleiro, Elena |e verfasserin |4 aut | |
| 700 | 1 | |a Pumarola, Tomàs |e verfasserin |4 aut | |
| 700 | 1 | |a Vall-Mayans, Martí |e verfasserin |4 aut | |
| 700 | 1 | |a Esperalba, Juliana |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Future microbiology |d 2006 |g 16(2021) vom: 09. Sept., Seite 967-976 |w (DE-627)NLM171766504 |x 1746-0921 |7 nnns |
| 773 | 1 | 8 | |g volume:16 |g year:2021 |g day:09 |g month:09 |g pages:967-976 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.2217/fmb-2021-0037 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 16 |j 2021 |b 09 |c 09 |h 967-976 | ||