Quantitative analysis of m6A RNA modification by LC-MS
© 2021 The Authors..
N6-adenosine methylation (m6A) of messenger RNA (mRNA) plays key regulatory roles in gene expression. Accurate measurement of m6A levels is thus critical to understand its dynamic changes in various biological settings. Here, we provide a protocol to quantitate the levels of adenosine and m6A in cellular mRNAs. Using nuclease and phosphatase, we digest mRNA into nucleosides, which are subsequently quantified using liquid chromatography mass spectrometry. For complete details on the use and execution of this protocol, please refer to Cho et al. (2021).
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:2 |
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| Enthalten in: |
STAR protocols - 2(2021), 3 vom: 17. Sept., Seite 100724 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Mathur, Lavina [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 21.03.2022 Date Revised 21.03.2022 published: Electronic-eCollection Citation Status MEDLINE |
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| doi: |
10.1016/j.xpro.2021.100724 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM329454137 |
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| 520 | |a N6-adenosine methylation (m6A) of messenger RNA (mRNA) plays key regulatory roles in gene expression. Accurate measurement of m6A levels is thus critical to understand its dynamic changes in various biological settings. Here, we provide a protocol to quantitate the levels of adenosine and m6A in cellular mRNAs. Using nuclease and phosphatase, we digest mRNA into nucleosides, which are subsequently quantified using liquid chromatography mass spectrometry. For complete details on the use and execution of this protocol, please refer to Cho et al. (2021) | ||
| 650 | 4 | |a Journal Article | |
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| 650 | 4 | |a cell culture | |
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| 700 | 1 | |a Jung, Sunhee |e verfasserin |4 aut | |
| 700 | 1 | |a Jang, Cholsoon |e verfasserin |4 aut | |
| 700 | 1 | |a Lee, Gina |e verfasserin |4 aut | |
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