Comparison of Freelite and N-Latex serum free light chain assays : a critical review
Croatian Society of Medical Biochemistry and Laboratory Medicine..
INTRODUCTION: The measurement of serum free light chain (FLC) represents a fundamental aspect on the assessment of patients with monoclonal gammopathies (MG). Different analytical methods for FLC have become available with the possibility to obtain different value with a substantial impact on the assessment of patients with MG. This study aimed to evaluate FLC results obtained with two different assays and how the difference value obtained can impact in the patient's assessment.
MATERIALS AND METHODS: Ninety-three patient serum samples that underwent analysis for FLC with two different methods, Serum Freelite (The Binding Site, Birmingham, UK) and N-Latex FLC (Siemens, Marburg, Germany), were included in this retrospective study. Statistical analysis was performed to evaluate correlation, difference, and the grade of concordance between the results obtained with the two methods.
RESULTS: Significant statistical differences between the results obtained from the two methods were found (P < 0.05). A good correlation was found (0.99 for κ FLC, 0.95 for λ FLC, and 0.94 for the κ/λ ratio, respectively). We found a weighted kappa value of 0.65 for κ/λ ratio, 0.65 for λ FLC and 0.90 for κ FLC. A positive bias found with the Bland-Altman plot mirrors overestimation of κ FLC and κ/λ ratio with Freelite compared to N-Latex, whilst a negative bias underscores underestimation of λ FLC by Freelite compared to N-Latex.
CONCLUSION: Although in general the concordance between Freelite and N-Latex appears satisfactory, several discrepancies could be evidenced and consequently the two assays are not interchangeable.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:31 |
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| Enthalten in: |
Biochemia medica - 31(2021), 3 vom: 15. Okt., Seite 030701 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Daves, Massimo [VerfasserIn] |
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| Links: |
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| Themen: |
Analytical techniques and equipment |
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| Anmerkungen: |
Date Completed 27.09.2021 Date Revised 25.04.2022 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.11613/BM.2021.030701 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM329373269 |
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| 100 | 1 | |a Daves, Massimo |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Comparison of Freelite and N-Latex serum free light chain assays |b a critical review |
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| 500 | |a Date Completed 27.09.2021 | ||
| 500 | |a Date Revised 25.04.2022 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Croatian Society of Medical Biochemistry and Laboratory Medicine. | ||
| 520 | |a INTRODUCTION: The measurement of serum free light chain (FLC) represents a fundamental aspect on the assessment of patients with monoclonal gammopathies (MG). Different analytical methods for FLC have become available with the possibility to obtain different value with a substantial impact on the assessment of patients with MG. This study aimed to evaluate FLC results obtained with two different assays and how the difference value obtained can impact in the patient's assessment | ||
| 520 | |a MATERIALS AND METHODS: Ninety-three patient serum samples that underwent analysis for FLC with two different methods, Serum Freelite (The Binding Site, Birmingham, UK) and N-Latex FLC (Siemens, Marburg, Germany), were included in this retrospective study. Statistical analysis was performed to evaluate correlation, difference, and the grade of concordance between the results obtained with the two methods | ||
| 520 | |a RESULTS: Significant statistical differences between the results obtained from the two methods were found (P < 0.05). A good correlation was found (0.99 for κ FLC, 0.95 for λ FLC, and 0.94 for the κ/λ ratio, respectively). We found a weighted kappa value of 0.65 for κ/λ ratio, 0.65 for λ FLC and 0.90 for κ FLC. A positive bias found with the Bland-Altman plot mirrors overestimation of κ FLC and κ/λ ratio with Freelite compared to N-Latex, whilst a negative bias underscores underestimation of λ FLC by Freelite compared to N-Latex | ||
| 520 | |a CONCLUSION: Although in general the concordance between Freelite and N-Latex appears satisfactory, several discrepancies could be evidenced and consequently the two assays are not interchangeable | ||
| 650 | 4 | |a Comparative Study | |
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a analytical techniques and equipment | |
| 650 | 4 | |a comparison | |
| 650 | 4 | |a free light chain | |
| 650 | 4 | |a immunoassays | |
| 650 | 4 | |a monoclonal gammopathies | |
| 650 | 7 | |a Immunoglobulin Light Chains |2 NLM | |
| 650 | 7 | |a Latex |2 NLM | |
| 700 | 1 | |a Piccin, Andrea |e verfasserin |4 aut | |
| 700 | 1 | |a Roccaforte, Vincenzo |e verfasserin |4 aut | |
| 700 | 1 | |a Lippi, Giuseppe |e verfasserin |4 aut | |
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