Chemical Protein Synthesis by Chemoselective #x03B1;-Ketoacid-Hydroxylamine (KAHA) Ligations with 5-Oxaproline
© 2021. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature..
Chemical protein synthesis enables the precise construction of proteins by employing solid-phase peptide synthesis and chemoselective ligations. One such chemoselective reaction suitable for protein synthesis is the α-Ketoacid-Hydroxylamine (KAHA) ligation. Fully unprotected peptides are ligated by a selective reaction between α-ketoacids and hydroxylamines to give native amide bonds. Herein, we describe the chemical synthesis of ubiquitin by a two-segment approach using the 5-oxaproline hydroxylamine.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2021 |
|---|---|
| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:2355 |
|---|---|
| Enthalten in: |
Methods in molecular biology (Clifton, N.J.) - 2355(2021) vom: 12., Seite 151-162 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Farnung, Jakob [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
α-Ketoacid |
|---|
| Anmerkungen: |
Date Completed 11.01.2022 Date Revised 11.01.2022 published: Print Citation Status MEDLINE |
|---|
| doi: |
10.1007/978-1-0716-1617-8_14 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM32930741X |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM32930741X | ||
| 003 | DE-627 | ||
| 005 | 20231225204830.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2021 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1007/978-1-0716-1617-8_14 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1097.xml |
| 035 | |a (DE-627)NLM32930741X | ||
| 035 | |a (NLM)34386958 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Farnung, Jakob |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Chemical Protein Synthesis by Chemoselective #x03B1;-Ketoacid-Hydroxylamine (KAHA) Ligations with 5-Oxaproline |
| 264 | 1 | |c 2021 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 11.01.2022 | ||
| 500 | |a Date Revised 11.01.2022 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2021. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature. | ||
| 520 | |a Chemical protein synthesis enables the precise construction of proteins by employing solid-phase peptide synthesis and chemoselective ligations. One such chemoselective reaction suitable for protein synthesis is the α-Ketoacid-Hydroxylamine (KAHA) ligation. Fully unprotected peptides are ligated by a selective reaction between α-ketoacids and hydroxylamines to give native amide bonds. Herein, we describe the chemical synthesis of ubiquitin by a two-segment approach using the 5-oxaproline hydroxylamine | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a 5-Oxaproline | |
| 650 | 4 | |a Hydroxylamine | |
| 650 | 4 | |a KAHA ligation | |
| 650 | 4 | |a SPPS | |
| 650 | 4 | |a Ubiquitin | |
| 650 | 4 | |a α-Ketoacid | |
| 650 | 7 | |a Hydroxylamines |2 NLM | |
| 650 | 7 | |a Keto Acids |2 NLM | |
| 650 | 7 | |a Proteins |2 NLM | |
| 650 | 7 | |a Hydroxylamine |2 NLM | |
| 650 | 7 | |a 2FP81O2L9Z |2 NLM | |
| 650 | 7 | |a 5-oxaproline |2 NLM | |
| 650 | 7 | |a 36839-09-5 |2 NLM | |
| 650 | 7 | |a Proline |2 NLM | |
| 650 | 7 | |a 9DLQ4CIU6V |2 NLM | |
| 700 | 1 | |a Song, Haewon |e verfasserin |4 aut | |
| 700 | 1 | |a Bode, Jeffrey W |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Methods in molecular biology (Clifton, N.J.) |d 1984 |g 2355(2021) vom: 12., Seite 151-162 |w (DE-627)NLM074849794 |x 1940-6029 |7 nnns |
| 773 | 1 | 8 | |g volume:2355 |g year:2021 |g day:12 |g pages:151-162 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1007/978-1-0716-1617-8_14 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 2355 |j 2021 |b 12 |h 151-162 | ||