The Zinc Transporter ZnuABC Is Critical for the Virulence of Chromobacterium violaceum and Contributes to Diverse Zinc-Dependent Physiological Processes
Chromobacterium violaceum is a ubiquitous environmental bacterium that causes sporadic life-threatening infections in humans. How C. violaceum acquires zinc to colonize environmental and host niches is unknown. In this work, we demonstrated that C. violaceum employs the zinc uptake system ZnuABC to overcome zinc limitation in the host, ensuring the zinc supply for several physiological demands. Our data indicated that the C. violaceum ZnuABC transporter is encoded in a zur-CV_RS15045-CV_RS15040-znuCBA operon. This operon was repressed by the zinc uptake regulator Zur and derepressed in the presence of the host protein calprotectin (CP) and the synthetic metal chelator EDTA. A ΔznuCBA mutant strain showed impaired growth under these zinc-chelated conditions. Moreover, the deletion of znuCBA provoked reductions in violacein production, swimming motility, biofilm formation, and bacterial competition. Remarkably, the ΔznuCBA mutant strain was highly attenuated for virulence in an in vivo mouse infection model and showed low capacities to colonize the liver, grow in the presence of CP, and resist neutrophil killing. Overall, our findings demonstrate that ZnuABC is essential for C. violaceum virulence, contributing to subversion of zinc-based host nutritional immunity.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:89 |
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| Enthalten in: |
Infection and immunity - 89(2021), 11 vom: 15. Okt., Seite e0031121 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Santos, Renato E R S [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 22.11.2021 Date Revised 16.04.2022 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1128/IAI.00311-21 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM329146386 |
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| 100 | 1 | |a Santos, Renato E R S |e verfasserin |4 aut | |
| 245 | 1 | 4 | |a The Zinc Transporter ZnuABC Is Critical for the Virulence of Chromobacterium violaceum and Contributes to Diverse Zinc-Dependent Physiological Processes |
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| 500 | |a Date Revised 16.04.2022 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Chromobacterium violaceum is a ubiquitous environmental bacterium that causes sporadic life-threatening infections in humans. How C. violaceum acquires zinc to colonize environmental and host niches is unknown. In this work, we demonstrated that C. violaceum employs the zinc uptake system ZnuABC to overcome zinc limitation in the host, ensuring the zinc supply for several physiological demands. Our data indicated that the C. violaceum ZnuABC transporter is encoded in a zur-CV_RS15045-CV_RS15040-znuCBA operon. This operon was repressed by the zinc uptake regulator Zur and derepressed in the presence of the host protein calprotectin (CP) and the synthetic metal chelator EDTA. A ΔznuCBA mutant strain showed impaired growth under these zinc-chelated conditions. Moreover, the deletion of znuCBA provoked reductions in violacein production, swimming motility, biofilm formation, and bacterial competition. Remarkably, the ΔznuCBA mutant strain was highly attenuated for virulence in an in vivo mouse infection model and showed low capacities to colonize the liver, grow in the presence of CP, and resist neutrophil killing. Overall, our findings demonstrate that ZnuABC is essential for C. violaceum virulence, contributing to subversion of zinc-based host nutritional immunity | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, N.I.H., Extramural | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Chromobacterium violaceum | |
| 650 | 4 | |a ZnuABC | |
| 650 | 4 | |a bacterial virulence | |
| 650 | 4 | |a bacterial zinc transporter | |
| 650 | 4 | |a calprotectin | |
| 650 | 4 | |a metalloregulator | |
| 650 | 4 | |a zinc homeostasis | |
| 650 | 4 | |a zinc uptake regulator | |
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| 650 | 7 | |a Leukocyte L1 Antigen Complex |2 NLM | |
| 650 | 7 | |a zinc-binding protein |2 NLM | |
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| 700 | 1 | |a da Silva Júnior, Waldir P |e verfasserin |4 aut | |
| 700 | 1 | |a Harrison, Simone |e verfasserin |4 aut | |
| 700 | 1 | |a Skaar, Eric P |e verfasserin |4 aut | |
| 700 | 1 | |a Chazin, Walter J |e verfasserin |4 aut | |
| 700 | 1 | |a da Silva Neto, José F |e verfasserin |4 aut | |
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