A highly polymorphic caprine keratin-associated protein gene identified and its effect on cashmere traits
© The Author(s) 2021. Published by Oxford University Press on behalf of the American Society of Animal Science. All rights reserved. For permissions, please e-mail: journals.permissionsoup.com..
Five keratin-associated protein 6 genes (KRTAP6) have been identified in sheep and variation in some KRTAP6 has been associated with wool fiber diameter-related traits, but none of these homologues have been identified in goats. In this study, we reported the identification of the sheep KRTAP6-5 homologue on goat chromosome 1 and polymerase chain reaction (PCR)-single strand conformation polymorphism analysis in 300 Longdong cashmere goats revealed the existence of 12 variant sequences. Both coding region and 3'UTR of the putative caprine KRTAP6-5 displayed a biggest sequence similarity to ovine KRTAP6-5 gene. This suggested that the gene represents caprine KRTAP6-5 sequences, and these sequences composed 23 genotypes, which was the most polymorphism gene in KRTAPs that have been studied. Among these sequences, 15 nucleotide substitutions and a 24-bp insertion/detection were identified. Variation in goat KRTAP6-5 was associated with variation in mean-fiber diameter, suggesting that KRTAP6-5 is worthy of further study in the context of variation in cashmere traits.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:99 |
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| Enthalten in: |
Journal of animal science - 99(2021), 9 vom: 01. Sept. |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Li, Shaobin [VerfasserIn] |
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| Links: |
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| Themen: |
68238-35-7 |
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| Anmerkungen: |
Date Completed 17.09.2021 Date Revised 11.08.2022 published: Print Citation Status MEDLINE |
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| doi: |
10.1093/jas/skab233 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM329141708 |
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| 245 | 1 | 2 | |a A highly polymorphic caprine keratin-associated protein gene identified and its effect on cashmere traits |
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| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © The Author(s) 2021. Published by Oxford University Press on behalf of the American Society of Animal Science. All rights reserved. For permissions, please e-mail: journals.permissionsoup.com. | ||
| 520 | |a Five keratin-associated protein 6 genes (KRTAP6) have been identified in sheep and variation in some KRTAP6 has been associated with wool fiber diameter-related traits, but none of these homologues have been identified in goats. In this study, we reported the identification of the sheep KRTAP6-5 homologue on goat chromosome 1 and polymerase chain reaction (PCR)-single strand conformation polymorphism analysis in 300 Longdong cashmere goats revealed the existence of 12 variant sequences. Both coding region and 3'UTR of the putative caprine KRTAP6-5 displayed a biggest sequence similarity to ovine KRTAP6-5 gene. This suggested that the gene represents caprine KRTAP6-5 sequences, and these sequences composed 23 genotypes, which was the most polymorphism gene in KRTAPs that have been studied. Among these sequences, 15 nucleotide substitutions and a 24-bp insertion/detection were identified. Variation in goat KRTAP6-5 was associated with variation in mean-fiber diameter, suggesting that KRTAP6-5 is worthy of further study in the context of variation in cashmere traits | ||
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| 700 | 1 | |a Zhao, Fangfang |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Jiqing |e verfasserin |4 aut | |
| 700 | 1 | |a He, Zhaohua |e verfasserin |4 aut | |
| 700 | 1 | |a Hu, Jiang |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Xiu |e verfasserin |4 aut | |
| 700 | 1 | |a Luo, Yuzhu |e verfasserin |4 aut | |
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