Remodeling and activation mechanisms of outer arm dyneins revealed by cryo-EM
© 2021 The Authors..
Cilia are thin microtubule-based protrusions of eukaryotic cells. The swimming of ciliated protists and sperm cells is propelled by the beating of cilia. Cilia propagate the flow of mucus in the trachea and protect the human body from viral infections. The main force generators of ciliary beating are the outer dynein arms (ODAs) which attach to the doublet microtubules. The bending of cilia is driven by the ODAs' conformational changes caused by ATP hydrolysis. Here, we report the native ODA complex structure attaching to the doublet microtubule by cryo-electron microscopy. The structure reveals how the ODA complex is attached to the doublet microtubule via the docking complex in its native state. Combined with coarse-grained molecular dynamic simulations, we present a model of how the attachment of the ODA to the doublet microtubule induces remodeling and activation of the ODA complex.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:22 |
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| Enthalten in: |
EMBO reports - 22(2021), 9 vom: 06. Sept., Seite e52911 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Kubo, Shintaroh [VerfasserIn] |
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| Links: |
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| Themen: |
Axonemal Dyneins |
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| Anmerkungen: |
Date Completed 22.10.2021 Date Revised 03.04.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.15252/embr.202152911 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM328829811 |
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| 245 | 1 | 0 | |a Remodeling and activation mechanisms of outer arm dyneins revealed by cryo-EM |
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| 500 | |a Date Revised 03.04.2024 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2021 The Authors. | ||
| 520 | |a Cilia are thin microtubule-based protrusions of eukaryotic cells. The swimming of ciliated protists and sperm cells is propelled by the beating of cilia. Cilia propagate the flow of mucus in the trachea and protect the human body from viral infections. The main force generators of ciliary beating are the outer dynein arms (ODAs) which attach to the doublet microtubules. The bending of cilia is driven by the ODAs' conformational changes caused by ATP hydrolysis. Here, we report the native ODA complex structure attaching to the doublet microtubule by cryo-electron microscopy. The structure reveals how the ODA complex is attached to the doublet microtubule via the docking complex in its native state. Combined with coarse-grained molecular dynamic simulations, we present a model of how the attachment of the ODA to the doublet microtubule induces remodeling and activation of the ODA complex | ||
| 650 | 4 | |a Journal Article | |
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| 650 | 4 | |a cryo-electron microscopy | |
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| 650 | 4 | |a outer arm dynein | |
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| 700 | 1 | |a Yang, Shun Kai |e verfasserin |4 aut | |
| 700 | 1 | |a Black, Corbin S |e verfasserin |4 aut | |
| 700 | 1 | |a Dai, Daniel |e verfasserin |4 aut | |
| 700 | 1 | |a Valente-Paterno, Melissa |e verfasserin |4 aut | |
| 700 | 1 | |a Gaertig, Jacek |e verfasserin |4 aut | |
| 700 | 1 | |a Ichikawa, Muneyoshi |e verfasserin |4 aut | |
| 700 | 1 | |a Bui, Khanh Huy |e verfasserin |4 aut | |
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