The role of antibody-based troponin detection in cardiovascular disease : A critical assessment
Copyright © 2021 Elsevier B.V. All rights reserved..
Cardiovascular disease has remained the world's biggest killer for 30 years. To aid in the diagnosis and prognosis of patients suffering cardiovascular-related disease accurate detection methods are essential. For over 20 years, the cardiac-specific troponins, I (cTnI) and T (cTnT), have acted as sensitive and specific biomarkers to assist in the diagnosis of various types of heart diseases. Various cardiovascular complications were commonly detected in patients with COVID-19, where cTn elevation is detectable, which suggested potential prognostic value of cTn in COVID-19-infected patients. Detection of these biomarkers circulating in the bloodstream is generally facilitated by immunoassays employing cTnI- and/or cTnT-specific antibodies. While several anti-troponin assays are commercially available, there are still obstacles to overcome to achieve optimal troponin detection. Such obstacles include the proteolytic degradation of N and C terminals on cTnI, epitope occlusion of troponin binding-sites by the cTnI/cTnT complex, cross reactivity of antibodies with skeletal troponins or assay interference caused by human anti-species antibodies. Therefore, further research into multi-antibody based platforms, multi-epitope targeting and rigorous validation of immunoassays is required to ensure accurate measurements. Moreover, in combination with various technical advances (e.g. microfluidics), antibody-based troponin detection systems can be more sensitive and rapid for incorporation into portable biosensor systems to be used at point-of care.
Medienart: |
E-Artikel |
---|
Erscheinungsjahr: |
2021 |
---|---|
Erschienen: |
2021 |
Enthalten in: |
Zur Gesamtaufnahme - volume:497 |
---|---|
Enthalten in: |
Journal of immunological methods - 497(2021) vom: 10. Okt., Seite 113108 |
Sprache: |
Englisch |
---|
Beteiligte Personen: |
Ma, Hui [VerfasserIn] |
---|
Links: |
---|
Themen: |
Antibodies |
---|
Anmerkungen: |
Date Completed 27.09.2021 Date Revised 07.01.2022 published: Print-Electronic Citation Status MEDLINE |
---|
doi: |
10.1016/j.jim.2021.113108 |
---|
funding: |
|
---|---|
Förderinstitution / Projekttitel: |
|
PPN (Katalog-ID): |
NLM328742791 |
---|
LEADER | 01000naa a22002652 4500 | ||
---|---|---|---|
001 | NLM328742791 | ||
003 | DE-627 | ||
005 | 20231225203606.0 | ||
007 | cr uuu---uuuuu | ||
008 | 231225s2021 xx |||||o 00| ||eng c | ||
024 | 7 | |a 10.1016/j.jim.2021.113108 |2 doi | |
028 | 5 | 2 | |a pubmed24n1095.xml |
035 | |a (DE-627)NLM328742791 | ||
035 | |a (NLM)34329690 | ||
035 | |a (PII)S0022-1759(21)00153-8 | ||
040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
041 | |a eng | ||
100 | 1 | |a Ma, Hui |e verfasserin |4 aut | |
245 | 1 | 4 | |a The role of antibody-based troponin detection in cardiovascular disease |b A critical assessment |
264 | 1 | |c 2021 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a ƒaComputermedien |b c |2 rdamedia | ||
338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
500 | |a Date Completed 27.09.2021 | ||
500 | |a Date Revised 07.01.2022 | ||
500 | |a published: Print-Electronic | ||
500 | |a Citation Status MEDLINE | ||
520 | |a Copyright © 2021 Elsevier B.V. All rights reserved. | ||
520 | |a Cardiovascular disease has remained the world's biggest killer for 30 years. To aid in the diagnosis and prognosis of patients suffering cardiovascular-related disease accurate detection methods are essential. For over 20 years, the cardiac-specific troponins, I (cTnI) and T (cTnT), have acted as sensitive and specific biomarkers to assist in the diagnosis of various types of heart diseases. Various cardiovascular complications were commonly detected in patients with COVID-19, where cTn elevation is detectable, which suggested potential prognostic value of cTn in COVID-19-infected patients. Detection of these biomarkers circulating in the bloodstream is generally facilitated by immunoassays employing cTnI- and/or cTnT-specific antibodies. While several anti-troponin assays are commercially available, there are still obstacles to overcome to achieve optimal troponin detection. Such obstacles include the proteolytic degradation of N and C terminals on cTnI, epitope occlusion of troponin binding-sites by the cTnI/cTnT complex, cross reactivity of antibodies with skeletal troponins or assay interference caused by human anti-species antibodies. Therefore, further research into multi-antibody based platforms, multi-epitope targeting and rigorous validation of immunoassays is required to ensure accurate measurements. Moreover, in combination with various technical advances (e.g. microfluidics), antibody-based troponin detection systems can be more sensitive and rapid for incorporation into portable biosensor systems to be used at point-of care | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a Research Support, Non-U.S. Gov't | |
650 | 4 | |a Review | |
650 | 4 | |a Antibody | |
650 | 4 | |a COVID-19 | |
650 | 4 | |a Cardiovascular disease | |
650 | 4 | |a Detection | |
650 | 4 | |a Troponin | |
650 | 7 | |a Antibodies |2 NLM | |
650 | 7 | |a Biomarkers |2 NLM | |
650 | 7 | |a Troponin I |2 NLM | |
650 | 7 | |a Troponin T |2 NLM | |
700 | 1 | |a Cassedy, Arabelle |e verfasserin |4 aut | |
700 | 1 | |a O'Kennedy, Richard |e verfasserin |4 aut | |
773 | 0 | 8 | |i Enthalten in |t Journal of immunological methods |d 1971 |g 497(2021) vom: 10. Okt., Seite 113108 |w (DE-627)NLM000031542 |x 1872-7905 |7 nnns |
773 | 1 | 8 | |g volume:497 |g year:2021 |g day:10 |g month:10 |g pages:113108 |
856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.jim.2021.113108 |3 Volltext |
912 | |a GBV_USEFLAG_A | ||
912 | |a GBV_NLM | ||
951 | |a AR | ||
952 | |d 497 |j 2021 |b 10 |c 10 |h 113108 |