Clinical and morphological characteristics of SARS-CoV-2-related myocarditis proven by the presence of viral RNA and proteins in myocardial tissue
OBJECTIVE: To investigate the clinical and morphological features of SARS-CoV-2-related myocarditis, by determining the presence of viral RNA and proteins in myocardial tissue.
MATERIAL AND METHODS: The study was conducted to examine the material of 32 autopsies with a confirmed diagnosis of myocarditis. There were data of a morphological study, including a standard histological study, as well as immunohistochemical determination of the surface markers CD45, CD3, CD20, and CD68 cells of an inflammatory infiltrate and virus proteins (SARS-CoV-2 nucleocapsid protein and spike protein). Positive and negative control tests were carried out. In addition, coronavirus RNA was detected in the myocardium using a polymerase chain reaction.
RESULTS: Polymerase chain reaction (PCR) revealed viral RNA in myocardial tissue. Viral proteins were identified in the macrophages of an inflammatory infiltrate and cardiomyocytes.
CONCLUSION: The findings may suggest that the virus persists in the myocardium and chronic myocarditis develops.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:83 |
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| Enthalten in: |
Arkhiv patologii - 83(2021), 4 vom: 19., Seite 5-13 |
| Sprache: |
Russisch |
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| Weiterer Titel: |
Kliniko-morfologicheskaya kharakteristika SARS-CoV-2-assotsiirovannogo miokardita, podtverzhdennogo nalichiem RNK i belkov virusa v tkani miokarda |
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| Beteiligte Personen: |
Kogan, E A [VerfasserIn] |
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| Links: |
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| Themen: |
COVID-19 |
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| Anmerkungen: |
Date Completed 20.07.2021 Date Revised 20.07.2021 published: Print Citation Status MEDLINE |
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| doi: |
10.17116/patol2021830415 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM328241091 |
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| 245 | 1 | 0 | |a Clinical and morphological characteristics of SARS-CoV-2-related myocarditis proven by the presence of viral RNA and proteins in myocardial tissue |
| 246 | 3 | 3 | |a Kliniko-morfologicheskaya kharakteristika SARS-CoV-2-assotsiirovannogo miokardita, podtverzhdennogo nalichiem RNK i belkov virusa v tkani miokarda |
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| 500 | |a Date Completed 20.07.2021 | ||
| 500 | |a Date Revised 20.07.2021 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a OBJECTIVE: To investigate the clinical and morphological features of SARS-CoV-2-related myocarditis, by determining the presence of viral RNA and proteins in myocardial tissue | ||
| 520 | |a MATERIAL AND METHODS: The study was conducted to examine the material of 32 autopsies with a confirmed diagnosis of myocarditis. There were data of a morphological study, including a standard histological study, as well as immunohistochemical determination of the surface markers CD45, CD3, CD20, and CD68 cells of an inflammatory infiltrate and virus proteins (SARS-CoV-2 nucleocapsid protein and spike protein). Positive and negative control tests were carried out. In addition, coronavirus RNA was detected in the myocardium using a polymerase chain reaction | ||
| 520 | |a RESULTS: Polymerase chain reaction (PCR) revealed viral RNA in myocardial tissue. Viral proteins were identified in the macrophages of an inflammatory infiltrate and cardiomyocytes | ||
| 520 | |a CONCLUSION: The findings may suggest that the virus persists in the myocardium and chronic myocarditis develops | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a COVID-19 | |
| 650 | 4 | |a SARS-CoV-2 RNA | |
| 650 | 4 | |a SARS-CoV-2 nucleocapsid protein | |
| 650 | 4 | |a SARS-CoV-2 spike protein | |
| 650 | 4 | |a coronavirus | |
| 650 | 4 | |a immunohistochemical examination | |
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| 650 | 4 | |a myocarditis | |
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| 700 | 1 | |a Zharkov, N V |e verfasserin |4 aut | |
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| 700 | 1 | |a Demyashkin, G A |e verfasserin |4 aut | |
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