Protocol for photoactivation of YAP in cancer cell spheroids embedded in collagen gels
© 2021 The Authors..
This protocol describes the necessary preparations and procedures to photo-activate Yes-associated protein (YAP) with optoYAP in cancer cell spheroids in 3D collagen matrices. We detail steps for immunofluorescent staining of the resulting YAP-activated HeLa spheroids. In addition, we describe handling of optoYAP on 2D substrates. While this protocol focuses on the use of optoYAP in 3D HeLa cell culture, it can be modified for other cell types. For complete details on the use and execution of this protocol, please refer to Illes et al. (2021).
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:2 |
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| Enthalten in: |
STAR protocols - 2(2021), 3 vom: 17. Sept., Seite 100657 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Illes, Bernhard [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 17.03.2022 Date Revised 17.03.2022 published: Electronic-eCollection Citation Status MEDLINE |
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| doi: |
10.1016/j.xpro.2021.100657 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM328236934 |
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| 520 | |a © 2021 The Authors. | ||
| 520 | |a This protocol describes the necessary preparations and procedures to photo-activate Yes-associated protein (YAP) with optoYAP in cancer cell spheroids in 3D collagen matrices. We detail steps for immunofluorescent staining of the resulting YAP-activated HeLa spheroids. In addition, we describe handling of optoYAP on 2D substrates. While this protocol focuses on the use of optoYAP in 3D HeLa cell culture, it can be modified for other cell types. For complete details on the use and execution of this protocol, please refer to Illes et al. (2021) | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Antibody | |
| 650 | 4 | |a Biotechnology and bioengineering | |
| 650 | 4 | |a Cancer | |
| 650 | 4 | |a Cell Biology | |
| 650 | 4 | |a Cell culture | |
| 650 | 4 | |a Microscopy | |
| 650 | 4 | |a Molecular/Chemical Probes | |
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