LncRNA-encoded microproteins : A new form of cargo in cell culture-derived and circulating extracellular vesicles
© 2021 The Authors. Journal of Extracellular Vesicles published by Wiley Periodicals, LLC on behalf of the International Society for Extracellular Vesicles..
Advancements in omics-based technologies over the past few years have led to the discovery of numerous biologically relevant peptides encoded by small open reading frames (smORFs) embedded in long noncoding RNA (lncRNA) transcripts (referred to as microproteins here) in a variety of species. However, the mechanisms and modes of action that underlie the roles of microproteins have yet to be fully characterized. Herein, we provide the first experimental evidence of abundant microproteins in extracellular vesicles (EVs) derived from glioma cancer cells, indicating that the EV-mediated transfer of microproteins may represent a novel mechanism for intercellular communication. Intriguingly, when examining human plasma, 48, 11 and 3 microproteins were identified from purified EVs, whole plasma and EV-free plasma, respectively, suggesting that circulating microproteins are primarily enriched in EVs. Most importantly, the preliminary data showed that the expression profile of EV microproteins in glioma patient diverged from the health donors, suggesting that the circulating microproteins in EVs might have potential diagnostic application in identifying patients with glioma.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:10 |
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| Enthalten in: |
Journal of extracellular vesicles - 10(2021), 9 vom: 24. Juli, Seite e12123 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Cai, Tanxi [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 09.02.2022 Date Revised 02.04.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1002/jev2.12123 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
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| 245 | 1 | 0 | |a LncRNA-encoded microproteins |b A new form of cargo in cell culture-derived and circulating extracellular vesicles |
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| 520 | |a © 2021 The Authors. Journal of Extracellular Vesicles published by Wiley Periodicals, LLC on behalf of the International Society for Extracellular Vesicles. | ||
| 520 | |a Advancements in omics-based technologies over the past few years have led to the discovery of numerous biologically relevant peptides encoded by small open reading frames (smORFs) embedded in long noncoding RNA (lncRNA) transcripts (referred to as microproteins here) in a variety of species. However, the mechanisms and modes of action that underlie the roles of microproteins have yet to be fully characterized. Herein, we provide the first experimental evidence of abundant microproteins in extracellular vesicles (EVs) derived from glioma cancer cells, indicating that the EV-mediated transfer of microproteins may represent a novel mechanism for intercellular communication. Intriguingly, when examining human plasma, 48, 11 and 3 microproteins were identified from purified EVs, whole plasma and EV-free plasma, respectively, suggesting that circulating microproteins are primarily enriched in EVs. Most importantly, the preliminary data showed that the expression profile of EV microproteins in glioma patient diverged from the health donors, suggesting that the circulating microproteins in EVs might have potential diagnostic application in identifying patients with glioma | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a cancer | |
| 650 | 4 | |a extracellular vesicles (EVs) | |
| 650 | 4 | |a lncRNA‐encoded microproteins | |
| 650 | 4 | |a long noncoding RNA (lncRNA) | |
| 650 | 4 | |a small open reading frames (smORFs) | |
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| 700 | 1 | |a Zhang, Qing |e verfasserin |4 aut | |
| 700 | 1 | |a Wu, Bowen |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Jifeng |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Na |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Tingting |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Zhipeng |e verfasserin |4 aut | |
| 700 | 1 | |a Luo, Jianjun |e verfasserin |4 aut | |
| 700 | 1 | |a Guo, Xiaojing |e verfasserin |4 aut | |
| 700 | 1 | |a Ding, Xiang |e verfasserin |4 aut | |
| 700 | 1 | |a Xie, Zhensheng |e verfasserin |4 aut | |
| 700 | 1 | |a Niu, Lili |e verfasserin |4 aut | |
| 700 | 1 | |a Ning, Weihai |e verfasserin |4 aut | |
| 700 | 1 | |a Fan, Zhen |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Xiaowei |e verfasserin |4 aut | |
| 700 | 1 | |a Guo, Xiangqian |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Runsheng |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Hongwei |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Fuquan |e verfasserin |4 aut | |
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