Tumor stem-like cells isolated from MMQ cells resist to dopamine agonist treatment
Copyright © 2021 Elsevier B.V. All rights reserved..
Although tumor stem-like cells (TSLCs) have been studied in a range of malignant tumors, evidence for the presence of these cells in pituitary adenomas needs further exploration. Here, we identified a small subset of sphere-forming cells possess tumor stem-like cell properties in rat prolactinoma MMQ cells, which resist to dopamine agonist treatment. Comparing to MMQ cells, sphere-forming cells showed higher cell viability after dopamine agonist (DA) treatment. Furthermore, the cells showed lower expression of prolactin (PRL) and dopamine 2 receptor (D2R). On the contrary, the daughter tumor cells differentiated from these cells restored the sensitivity to DA and showed high expression of PRL and D2R. The lower D2R expression and DA resistance might be due to DNA hypermethylation of D2R promoter. Our study demonstrates that the sphere-forming cells isolated from MMQ cells possess the trait of TSLCs and resist to DA treatment, which offers the opportunity to further investigate the mechanisms underlying tumor recurrence based on TSLCs.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:535 |
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| Enthalten in: |
Molecular and cellular endocrinology - 535(2021) vom: 15. Sept., Seite 111396 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Cai, Lin [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 25.01.2022 Date Revised 25.01.2022 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.mce.2021.111396 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM32816481X |
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| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2021 Elsevier B.V. All rights reserved. | ||
| 520 | |a Although tumor stem-like cells (TSLCs) have been studied in a range of malignant tumors, evidence for the presence of these cells in pituitary adenomas needs further exploration. Here, we identified a small subset of sphere-forming cells possess tumor stem-like cell properties in rat prolactinoma MMQ cells, which resist to dopamine agonist treatment. Comparing to MMQ cells, sphere-forming cells showed higher cell viability after dopamine agonist (DA) treatment. Furthermore, the cells showed lower expression of prolactin (PRL) and dopamine 2 receptor (D2R). On the contrary, the daughter tumor cells differentiated from these cells restored the sensitivity to DA and showed high expression of PRL and D2R. The lower D2R expression and DA resistance might be due to DNA hypermethylation of D2R promoter. Our study demonstrates that the sphere-forming cells isolated from MMQ cells possess the trait of TSLCs and resist to DA treatment, which offers the opportunity to further investigate the mechanisms underlying tumor recurrence based on TSLCs | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Dopamine 2 receptor | |
| 650 | 4 | |a Dopamine agonist | |
| 650 | 4 | |a Drug resistance | |
| 650 | 4 | |a MMQ cells | |
| 650 | 4 | |a Prolactinoma | |
| 650 | 4 | |a Tumor stem-like cells | |
| 650 | 7 | |a Dopamine Agonists |2 NLM | |
| 650 | 7 | |a Receptors, Dopamine D2 |2 NLM | |
| 650 | 7 | |a Prolactin |2 NLM | |
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| 700 | 1 | |a Chen, Jian |e verfasserin |4 aut | |
| 700 | 1 | |a Lu, Jianglong |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Qun |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Xianbin |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Linlin |e verfasserin |4 aut | |
| 700 | 1 | |a Wu, Jinsen |e verfasserin |4 aut | |
| 700 | 1 | |a Zheng, Weiming |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Chengde |e verfasserin |4 aut | |
| 700 | 1 | |a Su, Zhipeng |e verfasserin |4 aut | |
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