The Wnt/PCP formin Daam1 drives cell-cell adhesion during nephron development
Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved..
E-cadherin junctions facilitate assembly and disassembly of cell contacts that drive development and homeostasis of epithelial tissues. In this study, using Xenopus embryonic kidney and Madin-Darby canine kidney (MDCK) cells, we investigate the role of the Wnt/planar cell polarity (PCP) formin Daam1 (Dishevelled-associated activator of morphogenesis 1) in regulating E-cadherin-based intercellular adhesion. Using live imaging, we show that Daam1 localizes to newly formed cell contacts in the developing nephron. Furthermore, analyses of junctional filamentous actin (F-actin) upon Daam1 depletion indicate decreased microfilament localization and slowed turnover. We also show that Daam1 is necessary for efficient and timely localization of junctional E-cadherin, mediated by Daam1's formin homology domain 2 (FH2). Finally, we establish that Daam1 signaling promotes organized movement of renal cells. This study demonstrates that Daam1 formin junctional activity is critical for epithelial tissue organization.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:36 |
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| Enthalten in: |
Cell reports - 36(2021), 1 vom: 06. Juli, Seite 109340 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Krneta-Stankic, Vanja [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 10.02.2022 Date Revised 10.02.2022 published: Print Citation Status MEDLINE |
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| doi: |
10.1016/j.celrep.2021.109340 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 100 | 1 | |a Krneta-Stankic, Vanja |e verfasserin |4 aut | |
| 245 | 1 | 4 | |a The Wnt/PCP formin Daam1 drives cell-cell adhesion during nephron development |
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| 520 | |a Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved. | ||
| 520 | |a E-cadherin junctions facilitate assembly and disassembly of cell contacts that drive development and homeostasis of epithelial tissues. In this study, using Xenopus embryonic kidney and Madin-Darby canine kidney (MDCK) cells, we investigate the role of the Wnt/planar cell polarity (PCP) formin Daam1 (Dishevelled-associated activator of morphogenesis 1) in regulating E-cadherin-based intercellular adhesion. Using live imaging, we show that Daam1 localizes to newly formed cell contacts in the developing nephron. Furthermore, analyses of junctional filamentous actin (F-actin) upon Daam1 depletion indicate decreased microfilament localization and slowed turnover. We also show that Daam1 is necessary for efficient and timely localization of junctional E-cadherin, mediated by Daam1's formin homology domain 2 (FH2). Finally, we establish that Daam1 signaling promotes organized movement of renal cells. This study demonstrates that Daam1 formin junctional activity is critical for epithelial tissue organization | ||
| 650 | 4 | |a Journal Article | |
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| 700 | 1 | |a Paulucci-Holthauzen, Adriana |e verfasserin |4 aut | |
| 700 | 1 | |a Kloc, Malgorzata |e verfasserin |4 aut | |
| 700 | 1 | |a Gladden, Andrew B |e verfasserin |4 aut | |
| 700 | 1 | |a Miller, Rachel K |e verfasserin |4 aut | |
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