Optical Control of Genome Editing by Photoactivatable Cas9
The CRISPR-Cas9 system offers targeted genome manipulation with simplicity. Combining the CRISPR-Cas9 with optogenetics technology, we have engineered photoactivatable Cas9 to precisely control the genome sequence in a spatiotemporal manner. Here we provide a detailed protocol for optogenetic genome editing experiments using photoactivatable Cas9, including that for the generation of guide RNA vectors, light-mediated Cas9 activation, and quantification of genome editing efficiency in mammalian cells.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:2312 |
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| Enthalten in: |
Methods in molecular biology (Clifton, N.J.) - 2312(2021) vom: 05., Seite 225-233 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Otabe, Takahiro [VerfasserIn] |
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| Links: |
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| Themen: |
CRISPR-Associated Protein 9 |
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| Anmerkungen: |
Date Completed 11.08.2021 Date Revised 04.01.2024 published: Print Citation Status MEDLINE |
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| doi: |
10.1007/978-1-0716-1441-9_13 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a The CRISPR-Cas9 system offers targeted genome manipulation with simplicity. Combining the CRISPR-Cas9 with optogenetics technology, we have engineered photoactivatable Cas9 to precisely control the genome sequence in a spatiotemporal manner. Here we provide a detailed protocol for optogenetic genome editing experiments using photoactivatable Cas9, including that for the generation of guide RNA vectors, light-mediated Cas9 activation, and quantification of genome editing efficiency in mammalian cells | ||
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