Metabolomic signatures in elite cyclists : differential characterization of a seeming normal endocrine status regarding three serum hormones
INTRODUCTION: Serum phenotyping of elite cyclists regarding cortisol, IGF1 and testosterone is a way to detect endocrine disruptions possibly explained by exercise overload, non-balanced diet or by doping. This latter disruption-driven approach is supported by fundamental physiology although without any evidence of any metabolic markers.
OBJECTIVES: Serum samples were distributed through Low, High or Normal endocrine classes according to hormone concentration. A 1H NMR metabolomic study of 655 serum obtained in the context of the longitudinal medical follow-up of 253 subjects was performed to discriminate the three classes for every endocrine phenotype.
METHODS: An original processing algorithm was built which combined a partial-least squares-based orthogonal correction of metabolomic signals and a shrinkage discriminant analysis (SDA) to get satisfying classifications. An extended validation procedure was used to plan in larger size cohorts a minimal size to get a global prediction rate (GPR), i.e. the product of the three class prediction rates, higher than 99.9%.
RESULTS: Considering the 200 most SDA-informative variables, a sigmoidal fitting of the GPR gave estimates of a minimal sample size to 929, 2346 and 1408 for cortisol, IGF1 and testosterone, respectively. Analysis of outliers from cortisol and testosterone Normal classes outside the 97.5%-confidence limit of score prediction revealed possibly (i) an inadequate protein intake for outliers or (ii) an intake of dietary ergogenics, glycine or glutamine, which might explain the significant presence of heterogeneous metabolic profiles in a supposedly normal cyclists subgroup.
CONCLUSION: In a next validation metabolomics study of a so-sized cohort, anthropological, clinical and dietary metadata should be recorded in priority at the blood collection time to confirm these functional hypotheses.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2021 |
|---|---|
| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:17 |
|---|---|
| Enthalten in: |
Metabolomics : Official journal of the Metabolomic Society - 17(2021), 7 vom: 06. Juli, Seite 67 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Paris, Alain [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
3XMK78S47O |
|---|
| Anmerkungen: |
Date Completed 18.01.2022 Date Revised 18.01.2022 published: Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1007/s11306-021-01812-4 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM327745215 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM327745215 | ||
| 003 | DE-627 | ||
| 005 | 20231225201449.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2021 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1007/s11306-021-01812-4 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1092.xml |
| 035 | |a (DE-627)NLM327745215 | ||
| 035 | |a (NLM)34228178 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Paris, Alain |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Metabolomic signatures in elite cyclists |b differential characterization of a seeming normal endocrine status regarding three serum hormones |
| 264 | 1 | |c 2021 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 18.01.2022 | ||
| 500 | |a Date Revised 18.01.2022 | ||
| 500 | |a published: Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a INTRODUCTION: Serum phenotyping of elite cyclists regarding cortisol, IGF1 and testosterone is a way to detect endocrine disruptions possibly explained by exercise overload, non-balanced diet or by doping. This latter disruption-driven approach is supported by fundamental physiology although without any evidence of any metabolic markers | ||
| 520 | |a OBJECTIVES: Serum samples were distributed through Low, High or Normal endocrine classes according to hormone concentration. A 1H NMR metabolomic study of 655 serum obtained in the context of the longitudinal medical follow-up of 253 subjects was performed to discriminate the three classes for every endocrine phenotype | ||
| 520 | |a METHODS: An original processing algorithm was built which combined a partial-least squares-based orthogonal correction of metabolomic signals and a shrinkage discriminant analysis (SDA) to get satisfying classifications. An extended validation procedure was used to plan in larger size cohorts a minimal size to get a global prediction rate (GPR), i.e. the product of the three class prediction rates, higher than 99.9% | ||
| 520 | |a RESULTS: Considering the 200 most SDA-informative variables, a sigmoidal fitting of the GPR gave estimates of a minimal sample size to 929, 2346 and 1408 for cortisol, IGF1 and testosterone, respectively. Analysis of outliers from cortisol and testosterone Normal classes outside the 97.5%-confidence limit of score prediction revealed possibly (i) an inadequate protein intake for outliers or (ii) an intake of dietary ergogenics, glycine or glutamine, which might explain the significant presence of heterogeneous metabolic profiles in a supposedly normal cyclists subgroup | ||
| 520 | |a CONCLUSION: In a next validation metabolomics study of a so-sized cohort, anthropological, clinical and dietary metadata should be recorded in priority at the blood collection time to confirm these functional hypotheses | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Cortisol | |
| 650 | 4 | |a Diet | |
| 650 | 4 | |a Doping | |
| 650 | 4 | |a IGF1 | |
| 650 | 4 | |a SDA | |
| 650 | 4 | |a Testosterone | |
| 650 | 7 | |a Testosterone |2 NLM | |
| 650 | 7 | |a 3XMK78S47O |2 NLM | |
| 650 | 7 | |a Hydrocortisone |2 NLM | |
| 650 | 7 | |a WI4X0X7BPJ |2 NLM | |
| 700 | 1 | |a Labrador, Boris |e verfasserin |4 aut | |
| 700 | 1 | |a Lejeune, François-Xavier |e verfasserin |4 aut | |
| 700 | 1 | |a Canlet, Cécile |e verfasserin |4 aut | |
| 700 | 1 | |a Molina, Jérôme |e verfasserin |4 aut | |
| 700 | 1 | |a Guinot, Michel |e verfasserin |4 aut | |
| 700 | 1 | |a Mégret, Armand |e verfasserin |4 aut | |
| 700 | 1 | |a Rieu, Michel |e verfasserin |4 aut | |
| 700 | 1 | |a Thalabard, Jean-Christophe |e verfasserin |4 aut | |
| 700 | 1 | |a Le Bouc, Yves |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Metabolomics : Official journal of the Metabolomic Society |d 2005 |g 17(2021), 7 vom: 06. Juli, Seite 67 |w (DE-627)NLM162625987 |x 1573-3890 |7 nnns |
| 773 | 1 | 8 | |g volume:17 |g year:2021 |g number:7 |g day:06 |g month:07 |g pages:67 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1007/s11306-021-01812-4 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 17 |j 2021 |e 7 |b 06 |c 07 |h 67 | ||