Single-cell analyses reveal suppressive tumor microenvironment of human colorectal cancer
© 2021 The Authors. Clinical and Translational Medicine published by John Wiley & Sons Australia, Ltd on behalf of Shanghai Institute of Clinical Bioinformatics..
Profiling heterologous cell types within tumors is essential to decipher tumor microenvironment that shapes tumor progress and determines the outcome of therapeutic response. Here, we comprehensively characterized transcriptomes of 34,037 single cells obtained from 12 treatment-naïve patients with colorectal cancer. Our comprehensive evaluation revealed attenuated B-cell antigen presentation, distinct regulatory T-cell clusters with different origin and novel polyfunctional tumor associated macrophages associated with CRC. Moreover, we identified expanded XCL1+ T-cell clusters associated with tumor mutational burden high status. We further explored the underlying molecular mechanisms by profiling epigenetic landscape and inferring transcription factor motifs using single-cell ATAC-seq. Our dataset and analysis approaches herein provide a rich resource for further study of the impact of immune cells and translational research for human colorectal cancer.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:11 |
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| Enthalten in: |
Clinical and translational medicine - 11(2021), 6 vom: 14. Juni, Seite e422 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Mei, Yan [VerfasserIn] |
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| Links: |
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| Themen: |
Biomarkers, Tumor |
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| Anmerkungen: |
Date Completed 08.02.2022 Date Revised 02.04.2024 published: Print Citation Status MEDLINE |
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| doi: |
10.1002/ctm2.422 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM327323760 |
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| 520 | |a © 2021 The Authors. Clinical and Translational Medicine published by John Wiley & Sons Australia, Ltd on behalf of Shanghai Institute of Clinical Bioinformatics. | ||
| 520 | |a Profiling heterologous cell types within tumors is essential to decipher tumor microenvironment that shapes tumor progress and determines the outcome of therapeutic response. Here, we comprehensively characterized transcriptomes of 34,037 single cells obtained from 12 treatment-naïve patients with colorectal cancer. Our comprehensive evaluation revealed attenuated B-cell antigen presentation, distinct regulatory T-cell clusters with different origin and novel polyfunctional tumor associated macrophages associated with CRC. Moreover, we identified expanded XCL1+ T-cell clusters associated with tumor mutational burden high status. We further explored the underlying molecular mechanisms by profiling epigenetic landscape and inferring transcription factor motifs using single-cell ATAC-seq. Our dataset and analysis approaches herein provide a rich resource for further study of the impact of immune cells and translational research for human colorectal cancer | ||
| 650 | 4 | |a Journal Article | |
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| 700 | 1 | |a Hu, Hao |e verfasserin |4 aut | |
| 700 | 1 | |a Lu, Guanming |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Lingdan |e verfasserin |4 aut | |
| 700 | 1 | |a Sun, Zhun |e verfasserin |4 aut | |
| 700 | 1 | |a Lü, Mengdie |e verfasserin |4 aut | |
| 700 | 1 | |a Ma, Wenhui |e verfasserin |4 aut | |
| 700 | 1 | |a Jiang, Ting |e verfasserin |4 aut | |
| 700 | 1 | |a Gao, YuanHong |e verfasserin |4 aut | |
| 700 | 1 | |a Li, LiRen |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Gong |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Zifeng |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Hanjie |e verfasserin |4 aut | |
| 700 | 1 | |a Wu, Duojiao |e verfasserin |4 aut | |
| 700 | 1 | |a Zhou, Pinghong |e verfasserin |4 aut | |
| 700 | 1 | |a Leng, Qibin |e verfasserin |4 aut | |
| 700 | 1 | |a Jia, Guangshuai |e verfasserin |4 aut | |
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