Hepatotoxicity of copper sulfide nanoparticles towards hepatocyte spheroids using a novel multi-concave agarose chip method
Aim: To explore the hepatotoxicity of copper sulfide nanoparticles (CuSNPs) toward hepatocyte spheroids. Materials & methods: Other than the traditional agarose method to generate hepatocyte spheroids, we developed a multi-concave agarose chip (MCAC) method to investigate changes in hepatocyte viability, morphology, mitochondrial membrane potential, reactive oxygen species and hepatobiliary transporter by CuSNPs. Results: The MCAC method allowed a large number of spheroids to be obtained per sample. CuSNPs showed hepatotoxicity in vitro through a decrease in spheroid viability, albumin/urea production and glycogen deposition. CuSNPs also introduced hepatocyte spheroid injury through alteration of mitochondrial membrane potential and reactive oxygen species, that could be reversed by N-acetyl-l-cysteine. CuSNPs significantly decreased the activity of BSEP transporter by downregulating its mRNA and protein levels. Activity of the MRP2 transporter remained unchanged. Conclusion: We observed the hepatotoxicity of CuSNPs in vitro with associated mechanisms in an advanced 3D culture system.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:16 |
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| Enthalten in: |
Nanomedicine (London, England) - 16(2021), 17 vom: 06. Juli, Seite 1487-1504 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Jiang, Tianyan [VerfasserIn] |
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| Links: |
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| Themen: |
789U1901C5 |
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| Anmerkungen: |
Date Completed 04.08.2021 Date Revised 04.08.2021 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.2217/nnm-2021-0011 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM327315458 |
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| 245 | 1 | 0 | |a Hepatotoxicity of copper sulfide nanoparticles towards hepatocyte spheroids using a novel multi-concave agarose chip method |
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| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Aim: To explore the hepatotoxicity of copper sulfide nanoparticles (CuSNPs) toward hepatocyte spheroids. Materials & methods: Other than the traditional agarose method to generate hepatocyte spheroids, we developed a multi-concave agarose chip (MCAC) method to investigate changes in hepatocyte viability, morphology, mitochondrial membrane potential, reactive oxygen species and hepatobiliary transporter by CuSNPs. Results: The MCAC method allowed a large number of spheroids to be obtained per sample. CuSNPs showed hepatotoxicity in vitro through a decrease in spheroid viability, albumin/urea production and glycogen deposition. CuSNPs also introduced hepatocyte spheroid injury through alteration of mitochondrial membrane potential and reactive oxygen species, that could be reversed by N-acetyl-l-cysteine. CuSNPs significantly decreased the activity of BSEP transporter by downregulating its mRNA and protein levels. Activity of the MRP2 transporter remained unchanged. Conclusion: We observed the hepatotoxicity of CuSNPs in vitro with associated mechanisms in an advanced 3D culture system | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a copper sulfide nanoparticles | |
| 650 | 4 | |a hepatobiliary transporters | |
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| 650 | 7 | |a Copper |2 NLM | |
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| 700 | 1 | |a Guo, Haoxiang |e verfasserin |4 aut | |
| 700 | 1 | |a Xia, Ya-Nan |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Yun |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Dandan |e verfasserin |4 aut | |
| 700 | 1 | |a Pang, Guibin |e verfasserin |4 aut | |
| 700 | 1 | |a Feng, Yahui |e verfasserin |4 aut | |
| 700 | 1 | |a Yu, Huan |e verfasserin |4 aut | |
| 700 | 1 | |a Wu, Yanxian |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Shaodian |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Yangyun |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Yong |e verfasserin |4 aut | |
| 700 | 1 | |a Wen, Hairuo |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Leshuai W |e verfasserin |4 aut | |
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