Yeast adaptive response to acetic acid stress involves structural alterations and increased stiffness of the cell wall
This work describes a coordinate and comprehensive view on the time course of the alterations occurring at the level of the cell wall during adaptation of a yeast cell population to sudden exposure to a sub-lethal stress induced by acetic acid. Acetic acid is a major inhibitory compound in industrial bioprocesses and a widely used preservative in foods and beverages. Results indicate that yeast cell wall resistance to lyticase activity increases during acetic acid-induced growth latency, corresponding to yeast population adaptation to sudden exposure to this stress. This response correlates with: (i) increased cell stiffness, assessed by atomic force microscopy (AFM); (ii) increased content of cell wall β-glucans, assessed by fluorescence microscopy, and (iii) slight increase of the transcription level of the GAS1 gene encoding a β-1,3-glucanosyltransferase that leads to elongation of (1→3)-β-D-glucan chains. Collectively, results reinforce the notion that the adaptive yeast response to acetic acid stress involves a coordinate alteration of the cell wall at the biophysical and molecular levels. These alterations guarantee a robust adaptive response essential to limit the futile cycle associated to the re-entry of the toxic acid form after the active expulsion of acetate from the cell interior.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:11 |
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| Enthalten in: |
Scientific reports - 11(2021), 1 vom: 16. Juni, Seite 12652 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Ribeiro, Ricardo A [VerfasserIn] |
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| Links: |
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| Themen: |
Acetic Acid |
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| Anmerkungen: |
Date Completed 04.11.2021 Date Revised 02.04.2024 published: Electronic Citation Status MEDLINE |
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| doi: |
10.1038/s41598-021-92069-3 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM326829881 |
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| 245 | 1 | 0 | |a Yeast adaptive response to acetic acid stress involves structural alterations and increased stiffness of the cell wall |
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| 500 | |a Date Revised 02.04.2024 | ||
| 500 | |a published: Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a This work describes a coordinate and comprehensive view on the time course of the alterations occurring at the level of the cell wall during adaptation of a yeast cell population to sudden exposure to a sub-lethal stress induced by acetic acid. Acetic acid is a major inhibitory compound in industrial bioprocesses and a widely used preservative in foods and beverages. Results indicate that yeast cell wall resistance to lyticase activity increases during acetic acid-induced growth latency, corresponding to yeast population adaptation to sudden exposure to this stress. This response correlates with: (i) increased cell stiffness, assessed by atomic force microscopy (AFM); (ii) increased content of cell wall β-glucans, assessed by fluorescence microscopy, and (iii) slight increase of the transcription level of the GAS1 gene encoding a β-1,3-glucanosyltransferase that leads to elongation of (1→3)-β-D-glucan chains. Collectively, results reinforce the notion that the adaptive yeast response to acetic acid stress involves a coordinate alteration of the cell wall at the biophysical and molecular levels. These alterations guarantee a robust adaptive response essential to limit the futile cycle associated to the re-entry of the toxic acid form after the active expulsion of acetate from the cell interior | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a Saccharomyces cerevisiae Proteins |2 NLM | |
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| 650 | 7 | |a Acetic Acid |2 NLM | |
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| 700 | 1 | |a Vitorino, Miguel V |e verfasserin |4 aut | |
| 700 | 1 | |a Godinho, Cláudia P |e verfasserin |4 aut | |
| 700 | 1 | |a Bourbon-Melo, Nuno |e verfasserin |4 aut | |
| 700 | 1 | |a Robalo, Tiago T |e verfasserin |4 aut | |
| 700 | 1 | |a Fernandes, Fábio |e verfasserin |4 aut | |
| 700 | 1 | |a Rodrigues, Mário S |e verfasserin |4 aut | |
| 700 | 1 | |a Sá-Correia, Isabel |e verfasserin |4 aut | |
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