Characterization of hyperglycemia due to sub-chronic administration of red ginseng extract via comparative global proteomic analysis
Ginseng (Panax ginseng Meyer) is commonly used as an herbal remedy worldwide. Few studies have explored the possible physiological changes in the liver although patients often self-medicate with ginseng preparations, which may lead to exceeding the recommended dose for long-term administration. Here, we analyzed changes in the hepatic proteins of mouse livers using quantitative proteomics after sub-chronic administration of Korean red ginseng (KRG) extract (control group and 0.5, 1.0, and 2.0 g/kg KRG) using tandem mass tag (TMT) 6-plex technology. The 1.0 and 2.0 g/kg KRG groups exhibited signs of liver injury, including increased levels of aspartate transaminase (AST) and alanine aminotransferase (ALT) in the serum. Furthermore, serum glucose levels were significantly higher following KRG administration compared with the control group. Based on the upregulated proteins found in the proteomic analysis, we found that increased cystathionine beta-synthase (CBS) and cystathionine gamma-lyase (CSE) levels promoted greater hydrogen sulfide (H2S) synthesis in the liver. This investigation provides novel evidence that sub-chronic administration of KRG can elevate H2S production by increasing protein expression of CBS and CSE in the liver.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:11 |
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| Enthalten in: |
Scientific reports - 11(2021), 1 vom: 11. Juni, Seite 12374 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Na, Ann-Yae [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 28.10.2021 Date Revised 28.10.2021 published: Electronic Citation Status MEDLINE |
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| doi: |
10.1038/s41598-021-91664-8 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM326652329 |
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| 245 | 1 | 0 | |a Characterization of hyperglycemia due to sub-chronic administration of red ginseng extract via comparative global proteomic analysis |
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| 520 | |a Ginseng (Panax ginseng Meyer) is commonly used as an herbal remedy worldwide. Few studies have explored the possible physiological changes in the liver although patients often self-medicate with ginseng preparations, which may lead to exceeding the recommended dose for long-term administration. Here, we analyzed changes in the hepatic proteins of mouse livers using quantitative proteomics after sub-chronic administration of Korean red ginseng (KRG) extract (control group and 0.5, 1.0, and 2.0 g/kg KRG) using tandem mass tag (TMT) 6-plex technology. The 1.0 and 2.0 g/kg KRG groups exhibited signs of liver injury, including increased levels of aspartate transaminase (AST) and alanine aminotransferase (ALT) in the serum. Furthermore, serum glucose levels were significantly higher following KRG administration compared with the control group. Based on the upregulated proteins found in the proteomic analysis, we found that increased cystathionine beta-synthase (CBS) and cystathionine gamma-lyase (CSE) levels promoted greater hydrogen sulfide (H2S) synthesis in the liver. This investigation provides novel evidence that sub-chronic administration of KRG can elevate H2S production by increasing protein expression of CBS and CSE in the liver | ||
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| 700 | 1 | |a Jo, Jung Jae |e verfasserin |4 aut | |
| 700 | 1 | |a Kwon, Oh Kwang |e verfasserin |4 aut | |
| 700 | 1 | |a Cho, Piljoung |e verfasserin |4 aut | |
| 700 | 1 | |a Gao, Yan |e verfasserin |4 aut | |
| 700 | 1 | |a Kim, Ju-Hyun |e verfasserin |4 aut | |
| 700 | 1 | |a Kim, Kyu Min |e verfasserin |4 aut | |
| 700 | 1 | |a Ki, Sung Hwan |e verfasserin |4 aut | |
| 700 | 1 | |a Lee, Sangkyu |e verfasserin |4 aut | |
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