Distinct antigen uptake receptors route to the same storage compartments for cross-presentation in dendritic cells
© 2021 The Authors. Immunology published by John Wiley & Sons Ltd..
An exclusive feature of dendritic cells (DCs) is their capacity to present exogenous antigens by MHC class I molecules, called cross-presentation. Here, we show that protein antigen can be conserved in mature murine DCs for several days in a lysosome-like storage compartment, distinct from MHC class II and early endosomal compartments, as an internal source for the supply of MHC class I ligands. Using two different uptake routes via Fcγ receptors and C-type lectin receptors, we could show that antigens were routed towards the same endolysosomal compartments after 48 h. The antigen-containing compartments lacked co-expression of molecules involved in MHC class I processing and presentation including TAP and proteasome subunits as shown by single-cell imaging flow cytometry. Moreover, we observed the absence of cathepsin S but selective co-localization of active cathepsin X with protein antigen in the storage compartments. This indicates cathepsin S-independent antigen degradation and a novel but yet undefined role for cathepsin X in antigen processing and cross-presentation by DCs. In summary, our data suggest that these antigen-containing compartments in DCs can conserve protein antigens from different uptake routes and contribute to long-lasting antigen cross-presentation.
| Errataetall: |
CommentIn: Immunology. 2021 Nov;164(3):399-400. - PMID 34651319 |
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| Medienart: |
E-Artikel |
| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:164 |
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| Enthalten in: |
Immunology - 164(2021), 3 vom: 10. Nov., Seite 494-506 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Ho, Nataschja I [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 12.11.2021 Date Revised 12.11.2021 published: Print-Electronic CommentIn: Immunology. 2021 Nov;164(3):399-400. - PMID 34651319 Citation Status MEDLINE |
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| doi: |
10.1111/imm.13382 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM326586067 |
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| 245 | 1 | 0 | |a Distinct antigen uptake receptors route to the same storage compartments for cross-presentation in dendritic cells |
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| 500 | |a CommentIn: Immunology. 2021 Nov;164(3):399-400. - PMID 34651319 | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2021 The Authors. Immunology published by John Wiley & Sons Ltd. | ||
| 520 | |a An exclusive feature of dendritic cells (DCs) is their capacity to present exogenous antigens by MHC class I molecules, called cross-presentation. Here, we show that protein antigen can be conserved in mature murine DCs for several days in a lysosome-like storage compartment, distinct from MHC class II and early endosomal compartments, as an internal source for the supply of MHC class I ligands. Using two different uptake routes via Fcγ receptors and C-type lectin receptors, we could show that antigens were routed towards the same endolysosomal compartments after 48 h. The antigen-containing compartments lacked co-expression of molecules involved in MHC class I processing and presentation including TAP and proteasome subunits as shown by single-cell imaging flow cytometry. Moreover, we observed the absence of cathepsin S but selective co-localization of active cathepsin X with protein antigen in the storage compartments. This indicates cathepsin S-independent antigen degradation and a novel but yet undefined role for cathepsin X in antigen processing and cross-presentation by DCs. In summary, our data suggest that these antigen-containing compartments in DCs can conserve protein antigens from different uptake routes and contribute to long-lasting antigen cross-presentation | ||
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| 700 | 1 | |a Bos, Erik |e verfasserin |4 aut | |
| 700 | 1 | |a Koster, Abraham J |e verfasserin |4 aut | |
| 700 | 1 | |a Verdoes, Martijn |e verfasserin |4 aut | |
| 700 | 1 | |a van Kooyk, Yvette |e verfasserin |4 aut | |
| 700 | 1 | |a Ossendorp, Ferry |e verfasserin |4 aut | |
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