Serosurvey for equine piroplasms in horses and donkeys from North-Western Nigeria using IFAT and ELISA
Equine piroplasmosis is caused by apicomplexan parasites, namely, Babesia caballi and Theileria equi, which are transmitted to equids principally through ticks. To ascertain the exposure of equines to agents of equine piroplasms, we tested serum samples collected from horses (n = 272) and donkeys (n = 170) in North-Western Nigeria for the presence of antibodies against B. caballi and T. equi using IFAT and ELISA. The seroprevalence of T. equi in the horses determined using IFAT and ELISA was 48.89% and 45.96%, respectively, while for B. caballi, it was 6.3% and 0.4%, respectively. For T. equi, the seroprevalence based on IFAT and ELISA results in donkeys was 14.1% and 2.9%, respectively, while for B. caballi, the seroprevalence was 2.4% and 0.6%, respectively, for ELISA and IFAT. Mixed infection detected in the horses using IFAT and ELISA was 5.5% and 0.4%, respectively, while no mixed infection was observed in the donkeys. The seroprevalence of T. equi was significantly (P < .0001) higher than that of B. caballi in both horses and donkeys. Comparatively, the IFAT detected a greater number of piroplasm seropositive animals than ELISA, indicating a difference in their diagnostic accuracy. Findings from this study confirm the existence of equine piroplasms in both horses and donkeys in North-Western Nigeria and highlights the need for robust and effective control measures against the disease.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2021 |
|---|---|
| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:42 |
|---|---|
| Enthalten in: |
Journal of immunoassay & immunochemistry - 42(2021), 6 vom: 02. Nov., Seite 648-661 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Onyiche, ThankGod E [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Babesia caballi |
|---|
| Anmerkungen: |
Date Completed 04.03.2022 Date Revised 04.03.2022 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1080/15321819.2021.1935274 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM326457631 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM326457631 | ||
| 003 | DE-627 | ||
| 005 | 20231225194635.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2021 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1080/15321819.2021.1935274 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1088.xml |
| 035 | |a (DE-627)NLM326457631 | ||
| 035 | |a (NLM)34097577 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Onyiche, ThankGod E |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Serosurvey for equine piroplasms in horses and donkeys from North-Western Nigeria using IFAT and ELISA |
| 264 | 1 | |c 2021 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 04.03.2022 | ||
| 500 | |a Date Revised 04.03.2022 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Equine piroplasmosis is caused by apicomplexan parasites, namely, Babesia caballi and Theileria equi, which are transmitted to equids principally through ticks. To ascertain the exposure of equines to agents of equine piroplasms, we tested serum samples collected from horses (n = 272) and donkeys (n = 170) in North-Western Nigeria for the presence of antibodies against B. caballi and T. equi using IFAT and ELISA. The seroprevalence of T. equi in the horses determined using IFAT and ELISA was 48.89% and 45.96%, respectively, while for B. caballi, it was 6.3% and 0.4%, respectively. For T. equi, the seroprevalence based on IFAT and ELISA results in donkeys was 14.1% and 2.9%, respectively, while for B. caballi, the seroprevalence was 2.4% and 0.6%, respectively, for ELISA and IFAT. Mixed infection detected in the horses using IFAT and ELISA was 5.5% and 0.4%, respectively, while no mixed infection was observed in the donkeys. The seroprevalence of T. equi was significantly (P < .0001) higher than that of B. caballi in both horses and donkeys. Comparatively, the IFAT detected a greater number of piroplasm seropositive animals than ELISA, indicating a difference in their diagnostic accuracy. Findings from this study confirm the existence of equine piroplasms in both horses and donkeys in North-Western Nigeria and highlights the need for robust and effective control measures against the disease | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Babesia caballi | |
| 650 | 4 | |a ELISA | |
| 650 | 4 | |a Horses | |
| 650 | 4 | |a IFAT | |
| 650 | 4 | |a Nigeria | |
| 650 | 4 | |a Theileria equi | |
| 650 | 4 | |a donkeys | |
| 700 | 1 | |a Sivakumar, Thillaiampalam |e verfasserin |4 aut | |
| 700 | 1 | |a Tuvshintulga, Bumduuren |e verfasserin |4 aut | |
| 700 | 1 | |a Nugraha, Arifin Budiman |e verfasserin |4 aut | |
| 700 | 1 | |a Ahedor, Believe |e verfasserin |4 aut | |
| 700 | 1 | |a Mofokeng, Lehlohonolo |e verfasserin |4 aut | |
| 700 | 1 | |a Luka, Joshua |e verfasserin |4 aut | |
| 700 | 1 | |a Mohammed, Ali |e verfasserin |4 aut | |
| 700 | 1 | |a Mbaya, Albert W |e verfasserin |4 aut | |
| 700 | 1 | |a Biu, Abdullahi A |e verfasserin |4 aut | |
| 700 | 1 | |a Yokoyama, Naoaki |e verfasserin |4 aut | |
| 700 | 1 | |a Thekisoe, Oriel |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Journal of immunoassay & immunochemistry |d 2001 |g 42(2021), 6 vom: 02. Nov., Seite 648-661 |w (DE-627)NLM113877358 |x 1532-4230 |7 nnns |
| 773 | 1 | 8 | |g volume:42 |g year:2021 |g number:6 |g day:02 |g month:11 |g pages:648-661 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1080/15321819.2021.1935274 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 42 |j 2021 |e 6 |b 02 |c 11 |h 648-661 | ||