Identification of putative abdominal vibration-related genes through transcriptome analyses in the brown planthopper (Nilaparvata lugens)
Copyright © 2021 Elsevier Inc. All rights reserved..
The sexually mature female brown planthoppers (BPHs) send out abdominal vibration (AV) signals through the rice so that the males can obtain intraspecific, gender, and localization information to prepare for mating. Destroying vibration signals is an alternative biological method for pest control. However, the regulatory mechanism of AV in female BPHs remains elusive, which presents an obstacle to pest control. We observed that before mating female BHPs emitted abdominal vibration signals that disappeared immediately after mating and reappeared after 6 days. Therefore, ovarian and brain samples of female BPHs from Unmated-6h+ (with AV), Mated-6h- (without AV) and Mated-6d+ (with AV) individuals were collected for transcript analyses. By transcriptional sequencing analyses, 33 candidate genes that might involve in the regulation of female AV were obtained. After selecting 4 candidate genes of them for verification by RNA interference (RNAi), it was found that interference of juvenile hormone binding protein (JHBP) could greatly reduce the probability and frequency of AV for female BPHs. In general, this study identified AV-related candidate genes in female BPHs through transcriptome analyses and provided an important basis for future research on pest control in BPHs.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:39 |
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| Enthalten in: |
Comparative biochemistry and physiology. Part D, Genomics & proteomics - 39(2021) vom: 16. Sept., Seite 100856 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Su, Qin [VerfasserIn] |
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| Links: |
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| Themen: |
Apolipoprotein D |
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| Anmerkungen: |
Date Completed 24.09.2021 Date Revised 24.09.2021 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.cbd.2021.100856 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM326383050 |
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| 245 | 1 | 0 | |a Identification of putative abdominal vibration-related genes through transcriptome analyses in the brown planthopper (Nilaparvata lugens) |
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| 500 | |a Date Revised 24.09.2021 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2021 Elsevier Inc. All rights reserved. | ||
| 520 | |a The sexually mature female brown planthoppers (BPHs) send out abdominal vibration (AV) signals through the rice so that the males can obtain intraspecific, gender, and localization information to prepare for mating. Destroying vibration signals is an alternative biological method for pest control. However, the regulatory mechanism of AV in female BPHs remains elusive, which presents an obstacle to pest control. We observed that before mating female BHPs emitted abdominal vibration signals that disappeared immediately after mating and reappeared after 6 days. Therefore, ovarian and brain samples of female BPHs from Unmated-6h+ (with AV), Mated-6h- (without AV) and Mated-6d+ (with AV) individuals were collected for transcript analyses. By transcriptional sequencing analyses, 33 candidate genes that might involve in the regulation of female AV were obtained. After selecting 4 candidate genes of them for verification by RNA interference (RNAi), it was found that interference of juvenile hormone binding protein (JHBP) could greatly reduce the probability and frequency of AV for female BPHs. In general, this study identified AV-related candidate genes in female BPHs through transcriptome analyses and provided an important basis for future research on pest control in BPHs | ||
| 650 | 4 | |a Journal Article | |
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| 650 | 4 | |a Apolipoprotein D | |
| 650 | 4 | |a Brain | |
| 650 | 4 | |a Juvenile hormone binding protein | |
| 650 | 4 | |a Ovary | |
| 650 | 4 | |a Pest control | |
| 650 | 4 | |a RNAi | |
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| 700 | 1 | |a Li, Wan-Xue |e verfasserin |4 aut | |
| 700 | 1 | |a Sun, Jia-Wei |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Shi-Hui |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Wen-Qing |e verfasserin |4 aut | |
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