Unified platform for genetic and serological detection of COVID-19 with single-molecule technology
The COVID-19 pandemic raises the need for diverse diagnostic approaches to rapidly detect different stages of viral infection. The flexible and quantitative nature of single-molecule imaging technology renders it optimal for development of new diagnostic tools. Here we present a proof-of-concept for a single-molecule based, enzyme-free assay for detection of SARS-CoV-2. The unified platform we developed allows direct detection of the viral genetic material from patients' samples, as well as their immune response consisting of IgG and IgM antibodies. Thus, it establishes a platform for diagnostics of COVID-19, which could also be adjusted to diagnose additional pathogens.
| Errataetall: |
UpdateIn: PLoS One. 2021 Jul 26;16(7):e0255096. - PMID 34310620 |
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| Medienart: |
E-Artikel |
| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - year:2021 |
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| Enthalten in: |
medRxiv : the preprint server for health sciences - (2021) vom: 27. Juli |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Furth, Noa [VerfasserIn] |
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| Anmerkungen: |
Date Revised 14.01.2022 published: Electronic UpdateIn: PLoS One. 2021 Jul 26;16(7):e0255096. - PMID 34310620 Citation Status PubMed-not-MEDLINE |
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| doi: |
10.1101/2021.05.25.21257501 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM326238611 |
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| 520 | |a The COVID-19 pandemic raises the need for diverse diagnostic approaches to rapidly detect different stages of viral infection. The flexible and quantitative nature of single-molecule imaging technology renders it optimal for development of new diagnostic tools. Here we present a proof-of-concept for a single-molecule based, enzyme-free assay for detection of SARS-CoV-2. The unified platform we developed allows direct detection of the viral genetic material from patients' samples, as well as their immune response consisting of IgG and IgM antibodies. Thus, it establishes a platform for diagnostics of COVID-19, which could also be adjusted to diagnose additional pathogens | ||
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| 700 | 1 | |a Shilo, Shay |e verfasserin |4 aut | |
| 700 | 1 | |a Cohen, Niv |e verfasserin |4 aut | |
| 700 | 1 | |a Erez, Nir |e verfasserin |4 aut | |
| 700 | 1 | |a Fedyuk, Vadim |e verfasserin |4 aut | |
| 700 | 1 | |a Schrager, Alexander M |e verfasserin |4 aut | |
| 700 | 1 | |a Weinberger, Adina |e verfasserin |4 aut | |
| 700 | 1 | |a Dror, Amiel A |e verfasserin |4 aut | |
| 700 | 1 | |a Zigron, Asaf |e verfasserin |4 aut | |
| 700 | 1 | |a Shehadeh, Mona |e verfasserin |4 aut | |
| 700 | 1 | |a Sela, Eyal |e verfasserin |4 aut | |
| 700 | 1 | |a Srouji, Samer |e verfasserin |4 aut | |
| 700 | 1 | |a Amit, Sharon |e verfasserin |4 aut | |
| 700 | 1 | |a Levy, Itzchak |e verfasserin |4 aut | |
| 700 | 1 | |a Segal, Eran |e verfasserin |4 aut | |
| 700 | 1 | |a Dahan, Rony |e verfasserin |4 aut | |
| 700 | 1 | |a Jones, Dan |e verfasserin |4 aut | |
| 700 | 1 | |a Douek, Daniel C |e verfasserin |4 aut | |
| 700 | 1 | |a Shema, Efrat |e verfasserin |4 aut | |
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