Modulation of Cellular Response to Different Parameters of the Rotating Magnetic Field (RMF)-An In Vitro Wound Healing Study
Since the effect of MFs (magnetic fields) on various biological systems has been studied, different results have been obtained from an insignificant effect of weak MFs on the disruption of the circadian clock system. On the other hand, magnetic fields, electromagnetic fields, or electric fields are used in medicine. The presented study was conducted to determine whether a low-frequency RMF (rotating magnetic field) with different field parameters could evoke the cellular response in vitro and is possible to modulate the cellular response. The cellular metabolic activity, ROS and Ca2+ concentration levels, wound healing assay, and gene expression analyses were conducted to evaluate the effect of RMF. It was shown that different values of magnetic induction (B) and frequency (f) of RMF evoke a different response of cells, e.g., increase in the general metabolic activity may be associated with the increasing of ROS levels. The lower intracellular Ca2+ concentration (for 50 Hz) evoked the inability of cells to wound closure. It can be stated that the subtle balance in the ROS level is crucial in the wound for the effective healing process, and it is possible to modulate the cellular response to the RMF in the context of an in vitro wound healing.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:22 |
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| Enthalten in: |
International journal of molecular sciences - 22(2021), 11 vom: 28. Mai |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Jedrzejczak-Silicka, Magdalena [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 21.06.2021 Date Revised 02.04.2024 published: Electronic Citation Status MEDLINE |
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| doi: |
10.3390/ijms22115785 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM326198784 |
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| 245 | 1 | 0 | |a Modulation of Cellular Response to Different Parameters of the Rotating Magnetic Field (RMF)-An In Vitro Wound Healing Study |
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| 500 | |a Date Revised 02.04.2024 | ||
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| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Since the effect of MFs (magnetic fields) on various biological systems has been studied, different results have been obtained from an insignificant effect of weak MFs on the disruption of the circadian clock system. On the other hand, magnetic fields, electromagnetic fields, or electric fields are used in medicine. The presented study was conducted to determine whether a low-frequency RMF (rotating magnetic field) with different field parameters could evoke the cellular response in vitro and is possible to modulate the cellular response. The cellular metabolic activity, ROS and Ca2+ concentration levels, wound healing assay, and gene expression analyses were conducted to evaluate the effect of RMF. It was shown that different values of magnetic induction (B) and frequency (f) of RMF evoke a different response of cells, e.g., increase in the general metabolic activity may be associated with the increasing of ROS levels. The lower intracellular Ca2+ concentration (for 50 Hz) evoked the inability of cells to wound closure. It can be stated that the subtle balance in the ROS level is crucial in the wound for the effective healing process, and it is possible to modulate the cellular response to the RMF in the context of an in vitro wound healing | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Ca2+concentration level | |
| 650 | 4 | |a ROS level | |
| 650 | 4 | |a cellular metabolic activity | |
| 650 | 4 | |a fibroblasts | |
| 650 | 4 | |a frequency | |
| 650 | 4 | |a keratinocites | |
| 650 | 4 | |a magnetic induction | |
| 650 | 4 | |a rotating magnetic field | |
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| 700 | 1 | |a Kordas, Marian |e verfasserin |4 aut | |
| 700 | 1 | |a Konopacki, Maciej |e verfasserin |4 aut | |
| 700 | 1 | |a Rakoczy, Rafał |e verfasserin |4 aut | |
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