Single-cell brain atlas of Parkinson's disease mouse model
Copyright © 2021 The Authors. Published by Elsevier Ltd.. All rights reserved..
Parkinson's disease (PD) is a neurodegenerative disease, leading to the impairment of movement execution. PD pathogenesis has been largely investigated, either limited to bulk transcriptomic levels or at certain cell types, which failed to capture the cellular heterogeneity and intrinsic interplays among distinct cell types. Here, we report the application of single-nucleus RNA-seq on midbrain, striatum, and cerebellum of the α-syn-A53T mouse, a well-established PD mouse model, and matched controls, generating the first single cell transcriptomic atlas for the PD model mouse brain composed of 46,174 individual cells. Additionally, we comprehensively depicte the dysfunctions in PD pathology, covering the elevation of NF-κB activity, the alteration of ion channel components, the perturbation of protein homeostasis network, and the dysregulation of glutamatergic signaling. Notably, we identify a variety of cell types closely associated with PD risk genes. Taken together, our study provides valuable resources to systematically dissect the molecular mechanism of PD pathogenesis at the single-cell resolution, which facilitates the development of novel approaches for diagnosis and therapies against PD.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:48 |
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| Enthalten in: |
Journal of genetics and genomics = Yi chuan xue bao - 48(2021), 4 vom: 20. Apr., Seite 277-288 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Zhong, Jixing [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 18.01.2022 Date Revised 18.01.2022 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.jgg.2021.01.003 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM326008403 |
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| 520 | |a Copyright © 2021 The Authors. Published by Elsevier Ltd.. All rights reserved. | ||
| 520 | |a Parkinson's disease (PD) is a neurodegenerative disease, leading to the impairment of movement execution. PD pathogenesis has been largely investigated, either limited to bulk transcriptomic levels or at certain cell types, which failed to capture the cellular heterogeneity and intrinsic interplays among distinct cell types. Here, we report the application of single-nucleus RNA-seq on midbrain, striatum, and cerebellum of the α-syn-A53T mouse, a well-established PD mouse model, and matched controls, generating the first single cell transcriptomic atlas for the PD model mouse brain composed of 46,174 individual cells. Additionally, we comprehensively depicte the dysfunctions in PD pathology, covering the elevation of NF-κB activity, the alteration of ion channel components, the perturbation of protein homeostasis network, and the dysregulation of glutamatergic signaling. Notably, we identify a variety of cell types closely associated with PD risk genes. Taken together, our study provides valuable resources to systematically dissect the molecular mechanism of PD pathogenesis at the single-cell resolution, which facilitates the development of novel approaches for diagnosis and therapies against PD | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Neuroinflammtion | |
| 650 | 4 | |a Parkinson's disease | |
| 650 | 4 | |a Pathogenesis | |
| 650 | 4 | |a Single-nucleus RNA-Seq | |
| 650 | 4 | |a α-syn-A53T | |
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| 700 | 1 | |a Zhu, Jiacheng |e verfasserin |4 aut | |
| 700 | 1 | |a Wu, Weiying |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Ge |e verfasserin |4 aut | |
| 700 | 1 | |a Lin, Xiumei |e verfasserin |4 aut | |
| 700 | 1 | |a Liang, Langchao |e verfasserin |4 aut | |
| 700 | 1 | |a Chai, Chaochao |e verfasserin |4 aut | |
| 700 | 1 | |a Zeng, Yuying |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Feiyue |e verfasserin |4 aut | |
| 700 | 1 | |a Luo, Lihua |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Jiankang |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Fang |e verfasserin |4 aut | |
| 700 | 1 | |a Huang, Zhen |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Xiuqing |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Yu |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Hongde |e verfasserin |4 aut | |
| 700 | 1 | |a Qiu, Xin |e verfasserin |4 aut | |
| 700 | 1 | |a Tang, Shengping |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Dongsheng |e verfasserin |4 aut | |
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