Coordination of microbe-host homeostasis by crosstalk with plant innate immunity
Plants grown in natural soil are colonized by phylogenetically structured communities of microbes known as the microbiota. Individual microbes can activate microbe-associated molecular pattern (MAMP)-triggered immunity (MTI), which limits pathogen proliferation but curtails plant growth, a phenomenon known as the growth-defence trade-off. Here, we report that, in monoassociations, 41% (62 out of 151) of taxonomically diverse root bacterial commensals suppress Arabidopsis thaliana root growth inhibition (RGI) triggered by immune-stimulating MAMPs or damage-associated molecular patterns. Amplicon sequencing of bacterial 16S rRNA genes reveals that immune activation alters the profile of synthetic communities (SynComs) comprising RGI-non-suppressive strains, whereas the presence of RGI-suppressive strains attenuates this effect. Root colonization by SynComs with different complexities and RGI-suppressive activities alters the expression of 174 core host genes, with functions related to root development and nutrient transport. Furthermore, RGI-suppressive SynComs specifically downregulate a subset of immune-related genes. Precolonization of plants with RGI-suppressive SynComs, or mutation of one commensal-downregulated transcription factor, MYB15, renders the plants more susceptible to opportunistic Pseudomonas pathogens. Our results suggest that RGI-non-suppressive and RGI-suppressive root commensals modulate host susceptibility to pathogens by either eliciting or dampening MTI responses, respectively. This interplay buffers the plant immune system against pathogen perturbation and defence-associated growth inhibition, ultimately leading to commensal-host homeostasis.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:7 |
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| Enthalten in: |
Nature plants - 7(2021), 6 vom: 24. Juni, Seite 814-825 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Ma, Ka-Wai [VerfasserIn] |
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| Links: |
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| Themen: |
Arabidopsis Proteins |
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| Anmerkungen: |
Date Completed 16.08.2021 Date Revised 02.04.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1038/s41477-021-00920-2 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM325807485 |
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| 520 | |a Plants grown in natural soil are colonized by phylogenetically structured communities of microbes known as the microbiota. Individual microbes can activate microbe-associated molecular pattern (MAMP)-triggered immunity (MTI), which limits pathogen proliferation but curtails plant growth, a phenomenon known as the growth-defence trade-off. Here, we report that, in monoassociations, 41% (62 out of 151) of taxonomically diverse root bacterial commensals suppress Arabidopsis thaliana root growth inhibition (RGI) triggered by immune-stimulating MAMPs or damage-associated molecular patterns. Amplicon sequencing of bacterial 16S rRNA genes reveals that immune activation alters the profile of synthetic communities (SynComs) comprising RGI-non-suppressive strains, whereas the presence of RGI-suppressive strains attenuates this effect. Root colonization by SynComs with different complexities and RGI-suppressive activities alters the expression of 174 core host genes, with functions related to root development and nutrient transport. Furthermore, RGI-suppressive SynComs specifically downregulate a subset of immune-related genes. Precolonization of plants with RGI-suppressive SynComs, or mutation of one commensal-downregulated transcription factor, MYB15, renders the plants more susceptible to opportunistic Pseudomonas pathogens. Our results suggest that RGI-non-suppressive and RGI-suppressive root commensals modulate host susceptibility to pathogens by either eliciting or dampening MTI responses, respectively. This interplay buffers the plant immune system against pathogen perturbation and defence-associated growth inhibition, ultimately leading to commensal-host homeostasis | ||
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| 700 | 1 | |a Jia, Yong |e verfasserin |4 aut | |
| 700 | 1 | |a Ordon, Jana |e verfasserin |4 aut | |
| 700 | 1 | |a Copeland, Charles |e verfasserin |4 aut | |
| 700 | 1 | |a Emonet, Aurélia |e verfasserin |4 aut | |
| 700 | 1 | |a Geldner, Niko |e verfasserin |4 aut | |
| 700 | 1 | |a Guan, Rui |e verfasserin |4 aut | |
| 700 | 1 | |a Stolze, Sara Christina |e verfasserin |4 aut | |
| 700 | 1 | |a Nakagami, Hirofumi |e verfasserin |4 aut | |
| 700 | 1 | |a Garrido-Oter, Ruben |e verfasserin |4 aut | |
| 700 | 1 | |a Schulze-Lefert, Paul |e verfasserin |4 aut | |
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