Protocol for the derivation, culturing, and differentiation of human iPS-cell-derived neuroepithelial stem cells to study neural differentiation in vitro
© 2021 The Author(s)..
Here, we present a revised protocol to derive neuroepithelial stem (NES) cells from human induced pluripotent stem cells. NES cells can be further differentiated into a culture of neurons (90%) and glia (10%). We describe how to derive and maintain NES cells in culture and how to differentiate them. In addition, we show the potential use of NES cells to study the role of reactive oxygen species in neuronal differentiation and a guideline for NES cell transfection. For complete details on the use and execution of this protocol, please refer to Calvo-Garrido et al. (2019); Falk et al. (2012).
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:2 |
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| Enthalten in: |
STAR protocols - 2(2021), 2 vom: 18. Juni, Seite 100528 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Calvo-Garrido, Javier [VerfasserIn] |
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| Links: |
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| Themen: |
Cell Differentiation |
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| Anmerkungen: |
Date Completed 21.03.2022 Date Revised 21.03.2022 published: Electronic-eCollection Citation Status MEDLINE |
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| doi: |
10.1016/j.xpro.2021.100528 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM325767580 |
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| 245 | 1 | 0 | |a Protocol for the derivation, culturing, and differentiation of human iPS-cell-derived neuroepithelial stem cells to study neural differentiation in vitro |
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| 500 | |a Date Revised 21.03.2022 | ||
| 500 | |a published: Electronic-eCollection | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2021 The Author(s). | ||
| 520 | |a Here, we present a revised protocol to derive neuroepithelial stem (NES) cells from human induced pluripotent stem cells. NES cells can be further differentiated into a culture of neurons (90%) and glia (10%). We describe how to derive and maintain NES cells in culture and how to differentiate them. In addition, we show the potential use of NES cells to study the role of reactive oxygen species in neuronal differentiation and a guideline for NES cell transfection. For complete details on the use and execution of this protocol, please refer to Calvo-Garrido et al. (2019); Falk et al. (2012) | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Cell Differentiation | |
| 650 | 4 | |a Neuroscience | |
| 650 | 4 | |a Stem Cells | |
| 700 | 1 | |a Winn, Dania |e verfasserin |4 aut | |
| 700 | 1 | |a Maffezzini, Camilla |e verfasserin |4 aut | |
| 700 | 1 | |a Wedell, Anna |e verfasserin |4 aut | |
| 700 | 1 | |a Freyer, Christoph |e verfasserin |4 aut | |
| 700 | 1 | |a Falk, Anna |e verfasserin |4 aut | |
| 700 | 1 | |a Wredenberg, Anna |e verfasserin |4 aut | |
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