Bioinformatic and mouse model reveal the potential high vulnerability of Leydig cells on SARS-CoV-2
2021 Annals of Translational Medicine. All rights reserved..
BACKGROUND: The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was first reported in China and spread rapidly since the end of 2019. Previous studies have confirmed that SARS-CoV-2 infects host cells via binding to angiotensin converting enzyme II (ACE2).
METHODS: To explore the expression of ACE2 and the potential risk of infection in testis, we performed a bioinformatic analysis based on public databases, and conducted a pilot study using a mouse model. We also collected clinical follow-up date on male patients who had recovered from COVID-19 for 6 months.
RESULTS: The results showed that the RNA expression of ACE2 was higher in testis compared with other organs. Single-cell analysis and immunocytochemistry further indicated that Leydig cells were at risk of SARS-CoV-2 infection. Green fluorescence was only detected in the Leydig cells after intratesticular injection of pseudovirus SARS-CoV-2 in the mouse model. In the clinical follow-up, serum total testosterone level was statistically lower in patients who had recovered from COVID-19 compared with healthy men (P=0.010).
CONCLUSIONS: The findings of the present study indicate the potential vulnerability of Leydig cells. It is important to monitor the reproductive system and its complications in male COVID-19 patients. Further studies are still needed on SARS-CoV-2-associated reproductive complications.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:9 |
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| Enthalten in: |
Annals of translational medicine - 9(2021), 8 vom: 11. Apr., Seite 678 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Zhang, Jiawei [VerfasserIn] |
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| Links: |
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| Themen: |
Bioinformatic |
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| Anmerkungen: |
Date Revised 23.04.2022 published: Print Citation Status PubMed-not-MEDLINE |
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| doi: |
10.21037/atm-21-936 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM325387451 |
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| 245 | 1 | 0 | |a Bioinformatic and mouse model reveal the potential high vulnerability of Leydig cells on SARS-CoV-2 |
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| 500 | |a Date Revised 23.04.2022 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status PubMed-not-MEDLINE | ||
| 520 | |a 2021 Annals of Translational Medicine. All rights reserved. | ||
| 520 | |a BACKGROUND: The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was first reported in China and spread rapidly since the end of 2019. Previous studies have confirmed that SARS-CoV-2 infects host cells via binding to angiotensin converting enzyme II (ACE2) | ||
| 520 | |a METHODS: To explore the expression of ACE2 and the potential risk of infection in testis, we performed a bioinformatic analysis based on public databases, and conducted a pilot study using a mouse model. We also collected clinical follow-up date on male patients who had recovered from COVID-19 for 6 months | ||
| 520 | |a RESULTS: The results showed that the RNA expression of ACE2 was higher in testis compared with other organs. Single-cell analysis and immunocytochemistry further indicated that Leydig cells were at risk of SARS-CoV-2 infection. Green fluorescence was only detected in the Leydig cells after intratesticular injection of pseudovirus SARS-CoV-2 in the mouse model. In the clinical follow-up, serum total testosterone level was statistically lower in patients who had recovered from COVID-19 compared with healthy men (P=0.010) | ||
| 520 | |a CONCLUSIONS: The findings of the present study indicate the potential vulnerability of Leydig cells. It is important to monitor the reproductive system and its complications in male COVID-19 patients. Further studies are still needed on SARS-CoV-2-associated reproductive complications | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) | |
| 650 | 4 | |a bioinformatic | |
| 650 | 4 | |a reproductive system | |
| 650 | 4 | |a testis | |
| 700 | 1 | |a Wu, Yuqi |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Shulin |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Xiaobin |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Rui |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Xiangwei |e verfasserin |4 aut | |
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