A flow cytometric assay to detect platelet-activating antibodies in VITT after ChAdOx1 nCov-19 vaccination
© 2021 by The American Society of Hematology..
Vaccination is crucial in combatting the severe acute respiratory syndrome coronavirus 2 pandemic. The rare complication of thrombocytopenia and thrombotic complications at unusual sites after ChAdOx1 nCov-19 vaccination is caused by platelet-activating antibodies directed against platelet factor 4 (PF4). We present a widely applicable whole-blood standard flow cytometric assay to identify the pathogenic antibodies associated with vaccine-induced immune-mediated thrombotic thrombocytopenia (VITT) after ChAdOx1 nCov-19 vaccination. This assay will enable rapid diagnosis by many laboratories. This trial was registered at www.clinicaltrials.gov as #NCT04370119.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:137 |
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| Enthalten in: |
Blood - 137(2021), 26 vom: 01. Juli, Seite 3656-3659 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Handtke, Stefan [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 09.07.2021 Date Revised 13.10.2022 published: Print ClinicalTrials.gov: NCT04370119 Citation Status MEDLINE |
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| doi: |
10.1182/blood.2021012064 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM324985711 |
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| 245 | 1 | 2 | |a A flow cytometric assay to detect platelet-activating antibodies in VITT after ChAdOx1 nCov-19 vaccination |
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| 500 | |a ClinicalTrials.gov: NCT04370119 | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2021 by The American Society of Hematology. | ||
| 520 | |a Vaccination is crucial in combatting the severe acute respiratory syndrome coronavirus 2 pandemic. The rare complication of thrombocytopenia and thrombotic complications at unusual sites after ChAdOx1 nCov-19 vaccination is caused by platelet-activating antibodies directed against platelet factor 4 (PF4). We present a widely applicable whole-blood standard flow cytometric assay to identify the pathogenic antibodies associated with vaccine-induced immune-mediated thrombotic thrombocytopenia (VITT) after ChAdOx1 nCov-19 vaccination. This assay will enable rapid diagnosis by many laboratories. This trial was registered at www.clinicaltrials.gov as #NCT04370119 | ||
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| 700 | 1 | |a Wolff, Martina |e verfasserin |4 aut | |
| 700 | 1 | |a Zaninetti, Carlo |e verfasserin |4 aut | |
| 700 | 1 | |a Wesche, Jan |e verfasserin |4 aut | |
| 700 | 1 | |a Schönborn, Linda |e verfasserin |4 aut | |
| 700 | 1 | |a Aurich, Konstanze |e verfasserin |4 aut | |
| 700 | 1 | |a Ulm, Lena |e verfasserin |4 aut | |
| 700 | 1 | |a Hübner, Nils-Olaf |e verfasserin |4 aut | |
| 700 | 1 | |a Becker, Karsten |e verfasserin |4 aut | |
| 700 | 1 | |a Thiele, Thomas |e verfasserin |4 aut | |
| 700 | 1 | |a Greinacher, Andreas |e verfasserin |4 aut | |
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