Human ACE2-Functionalized Gold "Virus-Trap" Nanostructures for Accurate Capture of SARS-CoV-2 and Single-Virus SERS Detection
© The Author(s) 2021..
The current COVID-19 pandemic urges the extremely sensitive and prompt detection of SARS-CoV-2 virus. Here, we present a Human Angiotensin-converting-enzyme 2 (ACE2)-functionalized gold "virus traps" nanostructure as an extremely sensitive SERS biosensor, to selectively capture and rapidly detect S-protein expressed coronavirus, such as the current SARS-CoV-2 in the contaminated water, down to the single-virus level. Such a SERS sensor features extraordinary 106-fold virus enrichment originating from high-affinity of ACE2 with S protein as well as "virus-traps" composed of oblique gold nanoneedles, and 109-fold enhancement of Raman signals originating from multi-component SERS effects. Furthermore, the identification standard of virus signals is established by machine-learning and identification techniques, resulting in an especially low detection limit of 80 copies mL-1 for the simulated contaminated water by SARS-CoV-2 virus with complex circumstance as short as 5 min, which is of great significance for achieving real-time monitoring and early warning of coronavirus. Moreover, here-developed method can be used to establish the identification standard for future unknown coronavirus, and immediately enable extremely sensitive and rapid detection of novel virus.
Supplementary Information: The online version contains supplementary material available at 10.1007/s40820-021-00620-8.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:13 |
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| Enthalten in: |
Nano-micro letters - 13(2021) vom: 31. Dez., Seite 109 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Yang, Yong [VerfasserIn] |
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| Links: |
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| Themen: |
“Virus-trap” nanostructure |
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| Anmerkungen: |
Date Revised 28.03.2023 published: Electronic-eCollection Citation Status PubMed-not-MEDLINE |
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| doi: |
10.1007/s40820-021-00620-8 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM324234295 |
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| 500 | |a published: Electronic-eCollection | ||
| 500 | |a Citation Status PubMed-not-MEDLINE | ||
| 520 | |a © The Author(s) 2021. | ||
| 520 | |a The current COVID-19 pandemic urges the extremely sensitive and prompt detection of SARS-CoV-2 virus. Here, we present a Human Angiotensin-converting-enzyme 2 (ACE2)-functionalized gold "virus traps" nanostructure as an extremely sensitive SERS biosensor, to selectively capture and rapidly detect S-protein expressed coronavirus, such as the current SARS-CoV-2 in the contaminated water, down to the single-virus level. Such a SERS sensor features extraordinary 106-fold virus enrichment originating from high-affinity of ACE2 with S protein as well as "virus-traps" composed of oblique gold nanoneedles, and 109-fold enhancement of Raman signals originating from multi-component SERS effects. Furthermore, the identification standard of virus signals is established by machine-learning and identification techniques, resulting in an especially low detection limit of 80 copies mL-1 for the simulated contaminated water by SARS-CoV-2 virus with complex circumstance as short as 5 min, which is of great significance for achieving real-time monitoring and early warning of coronavirus. Moreover, here-developed method can be used to establish the identification standard for future unknown coronavirus, and immediately enable extremely sensitive and rapid detection of novel virus | ||
| 520 | |a Supplementary Information: The online version contains supplementary material available at 10.1007/s40820-021-00620-8 | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Human ACE2 | |
| 650 | 4 | |a SARS-CoV-2 | |
| 650 | 4 | |a SERS | |
| 650 | 4 | |a Single-virus detection | |
| 650 | 4 | |a “Virus-trap” nanostructure | |
| 700 | 1 | |a Peng, Yusi |e verfasserin |4 aut | |
| 700 | 1 | |a Lin, Chenglong |e verfasserin |4 aut | |
| 700 | 1 | |a Long, Li |e verfasserin |4 aut | |
| 700 | 1 | |a Hu, Jingying |e verfasserin |4 aut | |
| 700 | 1 | |a He, Jun |e verfasserin |4 aut | |
| 700 | 1 | |a Zeng, Hui |e verfasserin |4 aut | |
| 700 | 1 | |a Huang, Zhengren |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Zhi-Yuan |e verfasserin |4 aut | |
| 700 | 1 | |a Tanemura, Masaki |e verfasserin |4 aut | |
| 700 | 1 | |a Shi, Jianlin |e verfasserin |4 aut | |
| 700 | 1 | |a Lombardi, John R |e verfasserin |4 aut | |
| 700 | 1 | |a Luo, Xiaoying |e verfasserin |4 aut | |
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