Euploidy rates are not affected when embryos are cultured in a continuous (CCM) or sequential culture medium (SCM) : a sibling oocyte study
© 2021. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature..
PURPOSE: To determine if euploidy rates and embryo development differ when blastocysts are cultured in CCM or SCM.
METHOD: A single-center retrospective observational study was performed from September 2018 to March 2019. Patients [23-46 years] with at least four fresh mature oocytes (MII) without severe male factor infertility were included. Sibling MII were injected and cultured in Global®Total®LP (CCM) or Sage Quinn's Advantage® Cleavage and Blastocyst media (SCM) under 6% CO2, 5% O2, and 89% N2. Fertilization, cleavage, day (D) 5 blastulation, usable blastocyst (blastocysts biopsied/normally fertilized oocytes), and euploidy rates were recorded. Blastocysts were graded prior to trophectoderm (TE) biopsy on D5, 6, or 7 for genetic testing and mitochondrial DNA (mtDNA) quantification.
RESULTS: According to clinical practice, 1452 MII were randomly distributed: 751 in CCM and 701 in SCM. No differences were observed in fertilization and cleavages rates for CCM and SCM (77.4% vs 75.5%, p = 0.429 and 97.6% vs 99.1%, p = 0.094, respectively). Blastulation rate on D5 was higher in CCM (70.6% vs 62.2, p = 0.009); however, usable blastocyst rates were comparable (CCM: 58.3% vs SCM: 56.7%, p = 0.625). From a Poisson regression model adjusted for confounding factors, euploidy rates were not different between media (aOR = 1.18, [0.94-1.48], p = 0.157). Euploid blastocyst's mtDNA values were similar (CCM: 32.2, [30.5, 34.1] and SCM: 33.5, [31.8, 35.2], p = 0.345) and top-quality blastocysts (AA/BA) were increased in SCM (OR=1.04, [1.00-1.09], p = 0.037).
CONCLUSION: Under controlled in vitro conditions, euploidy rates and embryo development are comparable when embryos are cultured in CCM or SCM.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:38 |
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| Enthalten in: |
Journal of assisted reproduction and genetics - 38(2021), 8 vom: 09. Aug., Seite 2199-2207 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Abdala, Andrea [VerfasserIn] |
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| Links: |
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| Themen: |
Blastocyst |
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| Anmerkungen: |
Date Completed 24.01.2022 Date Revised 02.08.2022 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1007/s10815-021-02187-0 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM323894798 |
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| 245 | 1 | 0 | |a Euploidy rates are not affected when embryos are cultured in a continuous (CCM) or sequential culture medium (SCM) |b a sibling oocyte study |
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| 500 | |a Date Completed 24.01.2022 | ||
| 500 | |a Date Revised 02.08.2022 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2021. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature. | ||
| 520 | |a PURPOSE: To determine if euploidy rates and embryo development differ when blastocysts are cultured in CCM or SCM | ||
| 520 | |a METHOD: A single-center retrospective observational study was performed from September 2018 to March 2019. Patients [23-46 years] with at least four fresh mature oocytes (MII) without severe male factor infertility were included. Sibling MII were injected and cultured in Global®Total®LP (CCM) or Sage Quinn's Advantage® Cleavage and Blastocyst media (SCM) under 6% CO2, 5% O2, and 89% N2. Fertilization, cleavage, day (D) 5 blastulation, usable blastocyst (blastocysts biopsied/normally fertilized oocytes), and euploidy rates were recorded. Blastocysts were graded prior to trophectoderm (TE) biopsy on D5, 6, or 7 for genetic testing and mitochondrial DNA (mtDNA) quantification | ||
| 520 | |a RESULTS: According to clinical practice, 1452 MII were randomly distributed: 751 in CCM and 701 in SCM. No differences were observed in fertilization and cleavages rates for CCM and SCM (77.4% vs 75.5%, p = 0.429 and 97.6% vs 99.1%, p = 0.094, respectively). Blastulation rate on D5 was higher in CCM (70.6% vs 62.2, p = 0.009); however, usable blastocyst rates were comparable (CCM: 58.3% vs SCM: 56.7%, p = 0.625). From a Poisson regression model adjusted for confounding factors, euploidy rates were not different between media (aOR = 1.18, [0.94-1.48], p = 0.157). Euploid blastocyst's mtDNA values were similar (CCM: 32.2, [30.5, 34.1] and SCM: 33.5, [31.8, 35.2], p = 0.345) and top-quality blastocysts (AA/BA) were increased in SCM (OR=1.04, [1.00-1.09], p = 0.037) | ||
| 520 | |a CONCLUSION: Under controlled in vitro conditions, euploidy rates and embryo development are comparable when embryos are cultured in CCM or SCM | ||
| 650 | 4 | |a Clinical Trial | |
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Observational Study | |
| 650 | 4 | |a Blastocyst | |
| 650 | 4 | |a Continuous culture media | |
| 650 | 4 | |a Euploidy rates | |
| 650 | 4 | |a PGT-A | |
| 650 | 4 | |a Sequential culture media | |
| 650 | 4 | |a TE biopsy | |
| 700 | 1 | |a Elkhatib, Ibrahim |e verfasserin |4 aut | |
| 700 | 1 | |a Bayram, Aşina |e verfasserin |4 aut | |
| 700 | 1 | |a Arnanz, Ana |e verfasserin |4 aut | |
| 700 | 1 | |a El-Damen, Ahmed |e verfasserin |4 aut | |
| 700 | 1 | |a Melado, Laura |e verfasserin |4 aut | |
| 700 | 1 | |a Lawrenz, Barbara |e verfasserin |4 aut | |
| 700 | 1 | |a Coughlan, Carol |e verfasserin |4 aut | |
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| 700 | 1 | |a Fatemi, Human M |e verfasserin |4 aut | |
| 700 | 1 | |a De Munck, Neelke |e verfasserin |4 aut | |
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