Microbiological diagnosis of polymicrobial periprosthetic joint infection revealed superiority of investigated tissue samples compared to sonicate fluid generated from the implant surface
Copyright © 2021 The Authors. Published by Elsevier Ltd.. All rights reserved..
OBJECTIVES: In the microbiological diagnosis of periprosthetic joint infection (PJI), there is much discussion about the methodology of obtaining proper specimens, the processing technique, and suitable culture media. This retrospective study was conducted to analyse the accuracy of our culture techniques.
METHODS: Tissue samples and components from 258 patients after revision arthroplasty of the hip, knee, and shoulder were investigated, and the results of tissue cultures (TC) were compared to those of sonicate fluid cultures (SFC). Furthermore, an evaluation was performed of the influence of different culture media on the detection rate.
RESULTS: PJI was confirmed in 186 patients. The overall sensitivity of TC was no different to that of SFC (91.3% vs 90.8%, P = 1). In 153 cases (82.3%), TC and SFC showed concordant positive results. Results were discordant in 33 cases (17.7%). When differentiated according to the type of infection, TC showed significantly better results than SFC in detecting polymicrobial infections (97.0% vs 67.0%, P = 0.004). There were also significant differences between the culture media regarding the yield of microorganisms.
CONCLUSIONS: TC was more effective in detecting co-infections. The best results were obtained using both TC and SFC. The choice of culture media has a significant influence on the quality of results.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:106 |
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| Enthalten in: |
International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases - 106(2021) vom: 01. Mai, Seite 302-307 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Rieber, Heime [VerfasserIn] |
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| Links: |
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| Themen: |
Comparative Study |
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| Anmerkungen: |
Date Completed 02.06.2021 Date Revised 02.06.2021 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.ijid.2021.03.085 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM323749852 |
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| 100 | 1 | |a Rieber, Heime |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Microbiological diagnosis of polymicrobial periprosthetic joint infection revealed superiority of investigated tissue samples compared to sonicate fluid generated from the implant surface |
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| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2021 The Authors. Published by Elsevier Ltd.. All rights reserved. | ||
| 520 | |a OBJECTIVES: In the microbiological diagnosis of periprosthetic joint infection (PJI), there is much discussion about the methodology of obtaining proper specimens, the processing technique, and suitable culture media. This retrospective study was conducted to analyse the accuracy of our culture techniques | ||
| 520 | |a METHODS: Tissue samples and components from 258 patients after revision arthroplasty of the hip, knee, and shoulder were investigated, and the results of tissue cultures (TC) were compared to those of sonicate fluid cultures (SFC). Furthermore, an evaluation was performed of the influence of different culture media on the detection rate | ||
| 520 | |a RESULTS: PJI was confirmed in 186 patients. The overall sensitivity of TC was no different to that of SFC (91.3% vs 90.8%, P = 1). In 153 cases (82.3%), TC and SFC showed concordant positive results. Results were discordant in 33 cases (17.7%). When differentiated according to the type of infection, TC showed significantly better results than SFC in detecting polymicrobial infections (97.0% vs 67.0%, P = 0.004). There were also significant differences between the culture media regarding the yield of microorganisms | ||
| 520 | |a CONCLUSIONS: TC was more effective in detecting co-infections. The best results were obtained using both TC and SFC. The choice of culture media has a significant influence on the quality of results | ||
| 650 | 4 | |a Comparative Study | |
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Diagnostics | |
| 650 | 4 | |a Periprosthetic joint infection | |
| 650 | 4 | |a Sonicate fluid culture | |
| 650 | 4 | |a Tissue culture | |
| 700 | 1 | |a Frontzek, Andre |e verfasserin |4 aut | |
| 700 | 1 | |a Heinrich, Stephanie |e verfasserin |4 aut | |
| 700 | 1 | |a Breil-Wirth, Andreas |e verfasserin |4 aut | |
| 700 | 1 | |a Messler, Julian |e verfasserin |4 aut | |
| 700 | 1 | |a Hegermann, Stefan |e verfasserin |4 aut | |
| 700 | 1 | |a Ulatowski, Martin |e verfasserin |4 aut | |
| 700 | 1 | |a Koutras, Christos |e verfasserin |4 aut | |
| 700 | 1 | |a Steinheisser, Enno |e verfasserin |4 aut | |
| 700 | 1 | |a Kruppa, Thomas |e verfasserin |4 aut | |
| 700 | 1 | |a Fischer, Martin |e verfasserin |4 aut | |
| 700 | 1 | |a Hammer, Michael |e verfasserin |4 aut | |
| 700 | 1 | |a Mullahi, Arjan |e verfasserin |4 aut | |
| 700 | 1 | |a Morawietz, Thomas |e verfasserin |4 aut | |
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