Transcript Regulation of the Recoded Archaeal α-l-Fucosidase In Vivo

Genetic decoding is flexible, due to programmed deviation of the ribosomes from standard translational rules, globally termed "recoding". In Archaea, recoding has been unequivocally determined only for termination codon readthrough events that regulate the incorporation of the unusual amino acids selenocysteine and pyrrolysine, and for -1 programmed frameshifting that allow the expression of a fully functional α-l-fucosidase in the crenarchaeon Saccharolobus solfataricus, in which several functional interrupted genes have been identified. Increasing evidence suggests that the flexibility of the genetic code decoding could provide an evolutionary advantage in extreme conditions, therefore, the identification and study of interrupted genes in extremophilic Archaea could be important from an astrobiological point of view, providing new information on the origin and evolution of the genetic code and on the limits of life on Earth. In order to shed some light on the mechanism of programmed -1 frameshifting in Archaea, here we report, for the first time, on the analysis of the transcription of this recoded archaeal α-l-fucosidase and of its full-length mutant in different growth conditions in vivo. We found that only the wild type mRNA significantly increased in S. solfataricus after cold shock and in cells grown in minimal medium containing hydrolyzed xyloglucan as carbon source. Our results indicated that the increased level of fucA mRNA cannot be explained by transcript up-regulation alone. A different mechanism related to translation efficiency is discussed.

Medienart:

E-Artikel

Erscheinungsjahr:

2021

Erschienen:

2021

Enthalten in:

Zur Gesamtaufnahme - volume:26

Enthalten in:

Molecules (Basel, Switzerland) - 26(2021), 7 vom: 25. März

Sprache:

Englisch

Beteiligte Personen:

De Lise, Federica [VerfasserIn]
Iacono, Roberta [VerfasserIn]
Strazzulli, Andrea [VerfasserIn]
Giglio, Rosa [VerfasserIn]
Curci, Nicola [VerfasserIn]
Maurelli, Luisa [VerfasserIn]
Avino, Rosario [VerfasserIn]
Carandente, Antonio [VerfasserIn]
Caliro, Stefano [VerfasserIn]
Tortora, Alessandra [VerfasserIn]
Lorenzini, Fabio [VerfasserIn]
Di Donato, Paola [VerfasserIn]
Moracci, Marco [VerfasserIn]
Cobucci-Ponzano, Beatrice [VerfasserIn]

Links:

Volltext

Themen:

Alpha-L-Fucosidase
Archaea
Archaeal Proteins
EC 3.2.1.51
Extremophiles
Journal Article
Limits of life
Programmed frameshifting
Recoding

Anmerkungen:

Date Completed 12.05.2021

Date Revised 12.05.2021

published: Electronic

Citation Status MEDLINE

doi:

10.3390/molecules26071861

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM323616291