A newly established canine NK-type cell line and its cytotoxic properties
© 2021 John Wiley & Sons Ltd..
We established a canine natural killer (NK)-type cell line called CNK-89 derived from a dog with NK cell neoplasia. Immunophenotyping analysis showed positive staining for CD5, CD8, CD45, CD56, CD79a and NKp46, while negative for CD3, CD4, CD14, CD20, CD21, CD34, Thy1, IgG, IgM and MHCII. Polymerase chain reaction analysis revealed the presence of CD56, NKG2D, NKp30, NKp44, NKp46 and perforin, but the absence of CD16, Ly49 and granzyme B mRNA. Treating CNK-89 cells with IL-2 did not change the expression of activating receptors, TNFα and IFNγ secretion and cytotoxic activity, however, treatment with IL-12 alone or in combinations with IL-15, IL-18 and IL-21 caused an increase in granzyme B and CD16 mRNA, IFNγ secretion and cytotoxic properties of the CNK-89 cell line.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:19 |
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| Enthalten in: |
Veterinary and comparative oncology - 19(2021), 3 vom: 27. Sept., Seite 567-577 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Grudzien, Malgorzata [VerfasserIn] |
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| Links: |
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| Themen: |
EC 3.4.21.- |
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| Anmerkungen: |
Date Completed 25.01.2022 Date Revised 25.01.2022 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1111/vco.12695 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM323307558 |
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| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2021 John Wiley & Sons Ltd. | ||
| 520 | |a We established a canine natural killer (NK)-type cell line called CNK-89 derived from a dog with NK cell neoplasia. Immunophenotyping analysis showed positive staining for CD5, CD8, CD45, CD56, CD79a and NKp46, while negative for CD3, CD4, CD14, CD20, CD21, CD34, Thy1, IgG, IgM and MHCII. Polymerase chain reaction analysis revealed the presence of CD56, NKG2D, NKp30, NKp44, NKp46 and perforin, but the absence of CD16, Ly49 and granzyme B mRNA. Treating CNK-89 cells with IL-2 did not change the expression of activating receptors, TNFα and IFNγ secretion and cytotoxic activity, however, treatment with IL-12 alone or in combinations with IL-15, IL-18 and IL-21 caused an increase in granzyme B and CD16 mRNA, IFNγ secretion and cytotoxic properties of the CNK-89 cell line | ||
| 650 | 4 | |a Journal Article | |
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| 650 | 4 | |a innate immunity | |
| 650 | 4 | |a lymphocytes | |
| 650 | 4 | |a natural killer cells | |
| 650 | 4 | |a oncology | |
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| 700 | 1 | |a Kutkowska, Justyna |e verfasserin |4 aut | |
| 700 | 1 | |a Ziolo, Ewa |e verfasserin |4 aut | |
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| 700 | 1 | |a Strzadala, Leon |e verfasserin |4 aut | |
| 700 | 1 | |a Rapak, Andrzej |e verfasserin |4 aut | |
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