Knockdown of Aurora-A inhibits proliferation and promotes apoptosis of HepG2 hepatocellular carcinoma cells
Objective To investigate the effects of knockdown of Aurora-A gene on the proliferation and apoptosis of HepG2 human hepatocellular carcinoma cells. Methods Aurora-A short hairpin RNA (Aurora-A shRNA) was designed and Aurora-A shRNA lentiviral vector was constructed and packed, and then transfected into HepG2 cells. Aurora-A mRNA expression was detected by real-time quantitative PCR. Aurora-A protein expression and phosphorylation level were detected by Western blotting. Cell proliferation was tested by MTT assay. Cell apoptosis was analyzed by flow cytometry. Results The Aurora-A shRNA lentiviral vector was successfully constructed and Aurora-A protein phosphorylation level was significantly reduced in HepG2 cells transfected with the lentiviral vector. When Aurora-a was knocked down, the proliferation of HepG2 cells decreased and the apoptosis rate increased significantly. Conclusion Knockdown of Aurora-A can inhibit the proliferation and promote the apoptosis of HepG2 cells.
| Medienart: |
Artikel |
|---|
| Erscheinungsjahr: |
2021 |
|---|---|
| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:37 |
|---|---|
| Enthalten in: |
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology - 37(2021), 3 vom: 20. März, Seite 233-239 |
| Sprache: |
Chinesisch |
|---|
| Beteiligte Personen: |
Li, Zhongxian [VerfasserIn] |
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| Themen: |
AURKA protein, human |
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| Anmerkungen: |
Date Completed 29.03.2021 Date Revised 31.05.2022 published: Print Citation Status MEDLINE |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM323221807 |
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| 100 | 1 | |a Li, Zhongxian |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Knockdown of Aurora-A inhibits proliferation and promotes apoptosis of HepG2 hepatocellular carcinoma cells |
| 264 | 1 | |c 2021 | |
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| 500 | |a Date Completed 29.03.2021 | ||
| 500 | |a Date Revised 31.05.2022 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Objective To investigate the effects of knockdown of Aurora-A gene on the proliferation and apoptosis of HepG2 human hepatocellular carcinoma cells. Methods Aurora-A short hairpin RNA (Aurora-A shRNA) was designed and Aurora-A shRNA lentiviral vector was constructed and packed, and then transfected into HepG2 cells. Aurora-A mRNA expression was detected by real-time quantitative PCR. Aurora-A protein expression and phosphorylation level were detected by Western blotting. Cell proliferation was tested by MTT assay. Cell apoptosis was analyzed by flow cytometry. Results The Aurora-A shRNA lentiviral vector was successfully constructed and Aurora-A protein phosphorylation level was significantly reduced in HepG2 cells transfected with the lentiviral vector. When Aurora-a was knocked down, the proliferation of HepG2 cells decreased and the apoptosis rate increased significantly. Conclusion Knockdown of Aurora-A can inhibit the proliferation and promote the apoptosis of HepG2 cells | ||
| 650 | 4 | |a Journal Article | |
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| 650 | 7 | |a AURKA protein, human |2 NLM | |
| 650 | 7 | |a EC 2.7.11.1 |2 NLM | |
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| 700 | 1 | |a Liu, Bin |e verfasserin |4 aut | |
| 700 | 1 | |a Bu, Wenping |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Di |e verfasserin |4 aut | |
| 700 | 1 | |a Yan, Hui |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Qing |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology |d 2003 |g 37(2021), 3 vom: 20. März, Seite 233-239 |w (DE-627)NLM148235522 |x 1007-8738 |7 nnns |
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